Lu Yuanan, Yu Qigui, Zamzow Jill P, Wang Yun, Losey George S, Balazs George H, Nerurkar Vivek R, Yanagihara Richard
a Retrovirology Research Laboratory, Pacific Biomedical Research Center , University of Hawaii, Leahi Hospital , Atherton Building, 3675 Kilauea Avenue , Honolulu , Hawaii , 96816 , USA.
b Department of Zoology , University of Hawaii at Manoa , 2538 The Mall , Honolulu , Hawaii , 96822 , USA.
J Aquat Anim Health. 2000 Mar;12(1):58-63. doi: 10.1577/1548-8667(2000)012<0058:DOGTHS>2.0.CO;2.
Samples of DNA were prepared from various tissues and organs (including snout, gill, eye, brain, heart, liver, gut content, intestine, swim bladder, spleen, gallbladder, spinal cord, gonad, and muscle) of six healthy appearing reef cleaner fish, saddleback wrasses Thalassoma duperrey, captured from a cleaning station in North Kaneohe Bay, Oahu, Hawaii. The DNA samples were tested for evidence of green turtle herpesvirus infection by nested polymerase chain reaction (PCR). Green turtle herpesviral sequences were detected in snout (3/6), gill (2/6), and liver (1/6). All other tissues were negative. Except for a single nucleotide substitution (from A to G at position 48, resulting in a single amino acid change from isoleucine to methionine at position 16), the DNA sequences detected in the fish were identical to that of a newly reported green turtle herpesvirus. Although preliminary, these data represent the first evidence for an association of a herpesvirus with saddleback wrasse, suggesting that cleaner fish may serve as vectors or carriers for the transmission of the agent causing green turtle fibropapilloma.
从夏威夷瓦胡岛卡内奥赫湾北部一个清洁站捕获的6条外观健康的鞍背裂唇鱼（Thalassoma duperrey）身上，采集了来自各种组织和器官（包括吻部、鳃、眼睛、大脑、心脏、肝脏、肠道内容物、肠道、鱼鳔、脾脏、胆囊、脊髓、性腺和肌肉）的DNA样本。通过巢式聚合酶链反应（PCR）检测DNA样本是否存在绿海龟疱疹病毒感染的证据。在吻部（3/6）、鳃（2/6）和肝脏（1/6）中检测到绿海龟疱疹病毒序列。所有其他组织均为阴性。除了一个单核苷酸替换（第48位从A变为G，导致第16位的单个氨基酸从异亮氨酸变为甲硫氨酸）外，在鱼身上检测到的DNA序列与新报道的绿海龟疱疹病毒的序列相同。尽管这些数据是初步的，但它们代表了疱疹病毒与鞍背裂唇鱼关联的首个证据，表明清洁鱼可能是导致绿海龟纤维瘤的病原体传播的载体。
