Lu Y, Wang Y, Yu Q, Aguirre A A, Balazs G H, Nerurkar V R, Yanagihara R
Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu 96816, USA.
Arch Virol. 2000;145(9):1885-93. doi: 10.1007/s007050070063.
An alpha-herpesvirus has been associated recently with green turtle fibropapilloma (FP). To further clarify the role of this newfound green turtle herpesvirus (GTHV) in the pathogenesis of FP, various normal-appearing tissues and organs (including skin, eye, brain, heart, liver, spleen, intestine, lung, kidney, nerve, gonad, tongue, gall bladder, urinary bladder, thyroid and peripheral blood mononuclear cells (PBMC) from blood) and tumor tissues from 19 green turtles (Chelonia mydas) with FP, and tissues from three green turtles without FP, collected during 1997 to 1999 in the Hawaiian Islands, were tested for GTHV sequences by nested polymerase chain reaction (PCR), using GTHV-specific oligonuclotide primers. GTHV sequences were detected in all tumors (51/51) and most tissues (133/167) of tumored turtles. By contrast, such sequences were undetectable in tissues (0/28) of three non-tumored turtles. Analysis of GTHV sequences detected in different tissues and tumors revealed a low degree of genetic diversity (<1%). The wide distribution of this newfound herpesvirus in tumors and tissues of tumored green turtles and its absence in tissues of non-tumored turtles, argues for an etiologic role in FP.
最近发现一种α疱疹病毒与绿海龟纤维乳头瘤（FP）有关。为了进一步阐明这种新发现的绿海龟疱疹病毒（GTHV）在FP发病机制中的作用，我们使用GTHV特异性寡核苷酸引物，通过巢式聚合酶链反应（PCR）对1997年至1999年期间在夏威夷群岛采集的19只患有FP的绿海龟（蠵龟）的各种外观正常的组织和器官（包括皮肤、眼睛、大脑、心脏、肝脏、脾脏、肠道、肺、肾脏、神经、性腺、舌头、胆囊、膀胱、甲状腺以及血液中的外周血单核细胞（PBMC））、肿瘤组织，以及3只无FP的绿海龟的组织进行了GTHV序列检测。在所有肿瘤（51/51）以及患瘤海龟的大多数组织（133/167）中都检测到了GTHV序列。相比之下，在3只未患瘤海龟的组织（0/28）中未检测到此类序列。对在不同组织和肿瘤中检测到的GTHV序列进行分析，发现其遗传多样性程度较低（<1%）。这种新发现的疱疹病毒在患瘤绿海龟的肿瘤和组织中广泛分布，而在未患瘤海龟的组织中不存在，这表明它在FP的病因学中起作用。
