a Department of Studies in Microbiology , University of Mysore , Mysore , India.
b Department of Studies in Chemistry , Karnataka State Open University (KSOU) , Mysore , India.
Artif Cells Nanomed Biotechnol. 2018 Nov;46(7):1444-1451. doi: 10.1080/21691401.2017.1373655. Epub 2017 Sep 8.
In the present study, nanoparticles of gold, iron oxide and zinc oxide (ZnO) were studied for cytotoxicity in the colorectal cancer cell HT 29. The metallic nanoparticles in the range of <50 and <100 nm were screened for anticancer activity by MTT assay. The nanoparticles were tested at concentrations ranging from 0.5 to 50 µg/ml. Zinc oxide exhibited significant anti-cancer activity in comparison to other nanoparticles. It had an IC50 value of 17.12 µg/ml. The mechanism of action was studied by fluorescence microscopy with acridine orange, propidium iodide and DAPI staining techniques. The ROS production of ZnO nanoparticles was determined by DCFH-DA. The ZnO nanoparticles were conjugated with novel hydrophobic peptides and evaluated for anticancer activity. It was observed that the nanoparticles peptide complex showed better cytotoxicity than either peptide or nanoparticle alone. Thus, the ZnO nanoparticles tested in our study has anticancer activity against colon cancer cells. It can also be conjugated with peptides and used for targeting cancer cells with higher efficacy.
在本研究中，研究了金、氧化铁和氧化锌 (ZnO) 的纳米粒子对结直肠癌细胞 HT 29 的细胞毒性。通过 MTT 测定筛选了范围在 <50 和 <100nm 的金属纳米粒子的抗癌活性。纳米粒子在 0.5 至 50μg/ml 的浓度范围内进行了测试。与其他纳米粒子相比，氧化锌表现出显著的抗癌活性。它的 IC50 值为 17.12μg/ml。通过吖啶橙、碘化丙啶和 DAPI 染色技术的荧光显微镜研究了作用机制。通过 DCFH-DA 测定了 ZnO 纳米粒子的 ROS 产生。将 ZnO 纳米粒子与新型疏水性肽缀合，并评估其抗癌活性。结果表明，纳米粒子肽复合物的细胞毒性优于单独的肽或纳米粒子。因此，本研究中测试的 ZnO 纳米粒子对结肠癌细胞具有抗癌活性。它还可以与肽缀合，用于更有效地靶向癌细胞。
