Boehm T L, Hirth H P, Kornhuber B, Drahovsky D
Zentrum der Biologischen Chemie, Universität Frankfurt am Main, FRG.
Eur J Cancer Clin Oncol. 1987 Jun;23(6):623-9. doi: 10.1016/0277-5379(87)90257-4.
We have determined the prevalence of amplification and rearrangements for c-myc, c-myb, c-mos, bcr, c-abl, c-Ha-ras-1, c-N-ras, and c-K-ras-2 in a total of 51 cases of human leukaemia (19 patients with AML, 13 cases with CML, 14 cases with ALL, and 5 cases with CLL). Amplifications at a level of more than 2 two copies per haploid genome are apparently very rare and were found only once for c-myb in a c-ALL patient. Oncogene rearrangements were not found except for bcr, which was rearranged in all cases of CML, and 5 cases of ALL studied. Restriction fragment lengths polymorphisms (RFLPs) were also analysed. A previously described rare 5 kb EcoRI allele at the c-mos locus was absent in our patients. Rare alleles at the c-Ha-ras-1 locus were found to be significantly more prevalent in our patients than in a control group. Transfection experiments revealed no dominant transforming oncogenes in the tumour DNA of 3 patients carrying such rare alleles.
我们已确定了51例人类白血病（19例急性髓细胞白血病患者、13例慢性髓细胞白血病患者、14例急性淋巴细胞白血病患者和5例慢性淋巴细胞白血病患者）中c-myc、c-myb、c-mos、bcr、c-abl、c-Ha-ras-1、c-N-ras和c-K-ras-2基因扩增及重排的发生率。每个单倍体基因组超过2份拷贝水平的扩增显然非常罕见，仅在1例c-ALL患者的c-myb基因中发现过一次。除了在所有慢性髓细胞白血病病例以及所研究的5例急性淋巴细胞白血病病例中发生重排的bcr基因外，未发现癌基因重排。还分析了限制性片段长度多态性（RFLP）。我们的患者中不存在先前描述的位于c-mos基因座的罕见5 kb EcoRI等位基因。发现c-Ha-ras-1基因座的罕见等位基因在我们的患者中比在对照组中明显更普遍。转染实验显示，在携带此类罕见等位基因的3例患者的肿瘤DNA中未发现显性转化癌基因。
