Plasma Aided Biomedical Research Group (pabmed), Biomedical Engineering Division, Graduate School of Science and Technology, TOBB University of Economics and Technology, Ankara 06560, Turkey.
Plasma Aided Biomedical Research Group (pabmed), Biomedical Engineering Department, Engineering Faculty, TOBB University of Economics and Technology, Ankara 06560, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Dec 1;81:271-279. doi: 10.1016/j.msec.2017.08.003. Epub 2017 Aug 2.
In this study, a controlled drug release platform, amyloid-like bovine serum albumin (AL-BSA) with ampicillin sodium salt (amp), was developed. To develop this platform, 5%, 10%, and 20% (w/w) ratios of amp:BSA were used with electrospinning to prepare nanofibers with average diameters of 132±69, 159±60, and 179±42nm, respectively. Fourier transform infrared spectroscopy demonstrated that AL-BSA could entrap large amounts of drug inside the nanofibers, which was attributed to the antimicrobial activity of the released drug against Escherichia coli and Staphylococcus aureus. The amount of drug released was measured by UV-VIS spectrophotometry. The nanofibrous matrix of the electrospun membrane showed controlled release behavior in all samples. The transport mechanism was Fickian for the low ratio of amp:BSA (5% w:w). When the drug ratio was increased to >10% (w:w), thicker fiber structures formed, suggesting that the drug traveled a longer distance to reach the fiber surface; thus, the mechanism of transport shifted from Fickian to non-Fickian.
在这项研究中，开发了一种具有氨苄西林钠（amp）的控释药物释放平台，类似淀粉样的牛血清白蛋白（AL-BSA）。为了开发这个平台，使用了 5%、10%和 20%（w/w）的 amp:BSA 比例进行静电纺丝，以制备平均直径分别为 132±69、159±60 和 179±42nm 的纳米纤维。傅里叶变换红外光谱表明，AL-BSA 可以将大量药物包裹在纳米纤维内部，这归因于释放的药物对大肠杆菌和金黄色葡萄球菌的抗菌活性。通过紫外可见分光光度法测量药物的释放量。电纺膜的纳米纤维基质在所有样品中均表现出控释行为。当药物比例增加到 >10%（w/w）时，形成了较厚的纤维结构，这表明药物需要走更长的距离才能到达纤维表面；因此，传输机制从菲克扩散转变为非菲克扩散。
