Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, Dourados, MS, 79804-970, Brazil.
Federal University of Pernambuco, Av. Professor Moraes Rêgo, Recife, PE, 50670-901, Brazil.
Appl Biochem Biotechnol. 2018 Mar;184(3):869-884. doi: 10.1007/s12010-017-2589-y. Epub 2017 Sep 9.
Doxorubicin is an anticancer drug whose toxic effects on non-cancer cells are associated with increased oxidative stress. This study investigated the chemical composition, antioxidant activity of the methanolic extract of Schinus terebinthifolius Raddi leaves (MESL) as well as effects against doxorubicin-induced toxicity in human erythrocytes, K562 human erythroleukemia cells, and mouse hearts. The chemical composition indicated the presence of phenolic compounds, flavonoids, tannins, and ascorbic acid. MESL showed antioxidant activity by scavenging free radicals and inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent, and was able to increase the enzymatic activity of superoxide dismutase and glutathione peroxidase in human erythrocytes, without influencing the activity of enzyme catalase. The increase of oxidative hemolysis and malondialdehyde levels in erythrocytes incubated with doxorubicin was reduced by treatment with MESL. The cytotoxic activity of doxorubicin in erythroleukemia cells treated with MESL was unmodified. Additionally, the extract protected mice against the doxorubicin-induced cardiotoxicity. In conclusion, the MESL exhibits antioxidant activity, reducing doxorubicin-induced oxidative stress without changing the anticancer action of the drug, and protects against doxorubicin-induced cardiotoxicity. Hence, these findings suggest that these effects are via anti-oxidative by inhibiting free radicals, decreased oxidative stress, and increased antioxidant enzyme activity.
多柔比星是一种抗癌药物，其对非癌细胞的毒性作用与氧化应激增加有关。本研究调查了 Schinus terebinthifolius Raddi 叶甲醇提取物（MESL）的化学成分、抗氧化活性以及对人红细胞、K562 人红白血病细胞和小鼠心脏的多柔比星诱导毒性的影响。化学成分表明存在酚类化合物、类黄酮、单宁和抗坏血酸。MESL 通过清除自由基以及抑制与氧化剂孵育的人红细胞的溶血和脂质过氧化作用表现出抗氧化活性，并且能够增加人红细胞中超氧化物歧化酶和谷胱甘肽过氧化物酶的酶活性，而不影响酶过氧化氢酶的活性。用 MESL 处理可降低与多柔比星孵育的红细胞中氧化溶血和丙二醛水平的升高。用 MESL 处理的红白血病细胞中多柔比星的细胞毒性活性没有改变。此外，该提取物可预防小鼠多柔比星引起的心脏毒性。总之，MESL 表现出抗氧化活性，可减轻多柔比星诱导的氧化应激，而不改变药物的抗癌作用，并可预防多柔比星引起的心脏毒性。因此，这些发现表明这些作用是通过抑制自由基、降低氧化应激和增加抗氧化酶活性来实现的。
