Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados-Itahum, Km 12, Dourados, MS 79804-970, Brazil.
Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universitária 79070-900 Campo Grande, MS, Brazil.
Oxid Med Cell Longev. 2022 Nov 25;2022:8790810. doi: 10.1155/2022/8790810. eCollection 2022.
Obesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. , MEBr showed antioxidant activity in different methods: DPPH, ABTS, FRAP, iron-reducing power, inhibition of -carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life.
肥胖是一种全球性的流行病，与氧化应激和多种其他疾病的发展有关。（Bong.）Steud. 是一种原产于巴西塞拉多的药用植物，常用于治疗肥胖症。在这种情况下，我们研究了叶子的甲醇提取物（MEBr）的化学成分，并评估了其抗氧化活性及其对高脂肪饮食（HFD 60%）喂养的肥胖小鼠的预防和治疗作用。此外，还评估了 MEBr 的急性口服毒性。在 MEBr 中，鉴定出 17 种糖基化合物，包括杨梅素、槲皮素、山奈酚、咖啡酰、氰苷和megastigmane。结果表明，MEBr 在不同方法中均具有抗氧化活性：DPPH、ABTS、FRAP、铁还原能力、-胡萝卜素漂白抑制和 DNA 片段化抑制。在人红细胞中，MEBr 增加了抗氧化酶超氧化物歧化酶和过氧化氢酶的活性。在氧化应激下，MEBr 减少了红细胞的氧化溶血和丙二醛（MDA）水平的产生。急性给予 MEBr（2000mg/kg）的小鼠没有表现出毒性迹象。在 90 天的时间里，小鼠同时接受水或 MEBr 和高脂肪饮食（HFD）以诱导肥胖。在这个阶段，MEBr 能够减少皮下白色脂肪组织（WAT）的增加，并防止心脏和大脑中 MDA 的增加。在 HFD 诱导肥胖 180 天后，在治疗的最后 60 天（120-180 天）同时给予 MEBr 和 HFD 的小鼠（HFD-MEBr）与 HFD-Control 组相比，减少了腹膜后和肠系膜 WAT 沉积和心脏、肝脏、肾脏和大脑中的 MDA 水平。MEBr 的这些作用与给予 sibutramine（HFD-Sibutramine，2mg/kg）的小鼠相似。综合来看，这些结果表明，来自 的叶子的化合物影响控制氧化应激和预防及治疗肥胖症的作用。因此，与肥胖相关的氧化应激减少和身体成分调节，可有助于预防肥胖相关的合并症并提高生活质量。
