Eskandari V, Amirzargar A A, Mahmoudi M J, Rahnemoon Z, Rahmani F, Sadati S, Rahmati Z, Gorzin F, Hedayat M, Rezaei N
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Cardiology, Amir Alam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Ir J Med Sci. 2018 May;187(2):359-368. doi: 10.1007/s11845-017-1680-2. Epub 2017 Sep 9.
Evidence shows that proinflammatory cytokines are important determinants of assessment of severity and prognosis of chronic heart failure (CHF).
We investigated whether peripheral expression of the proinflammmatory factors, TNF-α and IL-6 can predict variable of clinical assessment of patients with CHF.
In this report, we used real-time PCR assay to compare relative gene expression of TNFα and IL-6 in PBMC from CHF patients with various heart diseases (n = 42, EF < 45%, NYHA I to IV) and matched healthy control subjects (n = 42).We also determined the TNFα and IL-6 concentrations of cell culture supernatant of PBMCs with ELISA.
There was a significant negative correlation between gene expression of TNFα and LVEF(r = 0.4, p < 0.05). Patients with CHF had increased gene expression of TNFα and IL-6 in PBMCs (p < 0.05). They also had elevated the supernatant levels of these cytokines in cultured PBMCs (p < 0.001). Levels of TNFα and IL-6 were increased in ischemic heart disease compared to non-ischemic heart disease. There was a positive correlation between TNFα and IL-6 levels in CHF patients and severity of CHF in patients. Levels of these cytokines were higher in patients with NYHA III-IV than in NYHA I-II and normal subjects.
Results of this study indicate that peripheral expression of proinflammatory cytokines, TNF-α and IL-6, is important indicators of severity and prognosis in patients with chronic heart disease.
有证据表明促炎细胞因子是慢性心力衰竭（CHF）严重程度和预后评估的重要决定因素。
我们研究了促炎因子肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的外周表达是否能够预测CHF患者临床评估的变量。
在本报告中，我们使用实时聚合酶链反应（PCR）检测法比较了各种心脏病CHF患者（n = 42，左心室射血分数[EF]<45%，纽约心脏协会心功能分级[NYHA]I至IV级）和匹配的健康对照者（n = 42）外周血单个核细胞（PBMC）中TNFα和IL-6的相对基因表达。我们还使用酶联免疫吸附测定（ELISA）法测定了PBMC细胞培养上清液中TNFα和IL-6的浓度。
TNFα基因表达与左心室射血分数呈显著负相关（r = 0.4，p < 0.05）。CHF患者PBMC中TNFα和IL-6的基因表达增加（p < 0.05）。他们培养的PBMC中这些细胞因子的上清液水平也升高（p < 0.001）。与非缺血性心脏病相比，缺血性心脏病中TNFα和IL-6的水平升高。CHF患者中TNFα和IL-6水平与CHF严重程度呈正相关。NYHA III-IV级患者中这些细胞因子的水平高于NYHA I-II级患者和正常受试者。
本研究结果表明，促炎细胞因子TNF-α和IL-6的外周表达是慢性心脏病患者严重程度和预后的重要指标。
