Rose Michael, Gaisa Nadine T
Institute of Pathology, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany.
Methods Mol Biol. 2018;1655:43-52. doi: 10.1007/978-1-4939-7234-0_4.
Immunohistochemistry is a standard method in histopathology and enables the localized detection of proteins in histological tissue sections. Specific antibodies are bound to cellular antigens, captured by secondary antibodies or polymers, linked to enzymes and visualized by chromogenic substrates. Here, we describe an automated staining technique for larger slide batches as well as a manual protocol for only few slides using a polymer technique. We focus on differentiation markers, measures of cell proliferation, and therapeutic targets in benign and malignant urothelial tissues.
免疫组织化学是组织病理学中的一种标准方法，可在组织学组织切片中对蛋白质进行定位检测。特异性抗体与细胞抗原结合，由二抗或聚合物捕获，连接到酶上，并通过显色底物进行可视化。在此，我们描述了一种针对较大批量玻片的自动染色技术以及一种使用聚合物技术对少量玻片的手动操作流程。我们关注良性和恶性尿路上皮组织中的分化标志物、细胞增殖指标以及治疗靶点。
