Hiruma Shota, Kamasaki Tomoko, Otomo Kohei, Nemoto Tomomi, Uehara Ryota
Graduate School of Life Science, Hokkaido University, Japan.
Institute for Genetic Medicine, Hokkaido University, Japan.
FEBS Lett. 2017 Oct;591(20):3296-3309. doi: 10.1002/1873-3468.12844. Epub 2017 Sep 20.
The molecular mechanism that governs cytoskeleton-membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane-associated domain in a cholesterol-dependent but largely Rho-independent manner. While ERMs are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted, ERMs increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site.
在动物细胞分裂过程中，调控细胞骨架与细胞膜相互作用的分子机制仍不清楚。在此，我们研究了ERM（埃兹蛋白/根蛋白/膜突蛋白）家族蛋白在人类培养细胞有丝分裂过程中的动态变化及功能。我们发现，埃兹蛋白通过其膜相关结构域以胆固醇依赖性但很大程度上不依赖Rho的方式被招募到分裂沟处。虽然ERM家族蛋白对于沟陷的形成并非必需，但它们在极性皮质的收缩活动中起关键作用。值得注意的是，当锚蛋白和 supervillin共同缺失时，ERM家族蛋白会在分裂沟处逐渐积累，并对沟陷的形成有显著贡献。这些结果揭示了ERM家族蛋白在细胞分裂过程中对皮质活动的支持作用，也为优先将与细胞分裂相关的蛋白与分裂位点联系起来的选择机制提供了见解。
