新型Cry50Ba杀蚊毒素的特性及其与其他杀蚊毒素的潜在协同作用
Characterization of a novel mosquitocidal toxin of Cry50Ba and its potential synergism with other mosquitocidal toxins.
作者信息
Zhang Wenfei, Yu Silan, Peng Silun, Gong Jianru, Qian Jiangzhao, He Jianqiao, Dai Wenyu, Wang Ruiping
机构信息
Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China.
出版信息
Toxicon. 2017 Nov;138:165-168. doi: 10.1016/j.toxicon.2017.08.025. Epub 2017 Sep 7.
A putative toxin gene of cry50Ba was successfully expressed in E. coli cells and confirmed that the purified Cry50Ba toxin had very high toxic activity against Culex quinquefasciatus larvae. Furthermore, the potential synergism of Cry50Ba toxin with Cry2Aa, Cry4Aa and Cry11Aa at a ratio of 1:1 was investigated. Although no significant synergism with other toxins was observed, the Cry50Ba as a novel toxin could be used to delay rapid onset of resistance in mosquito.
cry50Ba的一个假定毒素基因在大肠杆菌细胞中成功表达，并且证实纯化的Cry50Ba毒素对致倦库蚊幼虫具有非常高的毒性活性。此外，还研究了Cry50Ba毒素与Cry2Aa、Cry4Aa和Cry11Aa以1:1比例的潜在协同作用。尽管未观察到与其他毒素有显著的协同作用，但Cry50Ba作为一种新型毒素可用于延缓蚊子抗药性的快速产生。
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