Zhang Wenfei, Zhang Jie, Crickmore Neil, Wu Zhongqi, Yang Yiran, Qian Jiangzhao, Wu Hongping, Wang Ruiping, Fang Xuanjun
Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China,
World J Microbiol Biotechnol. 2014 Dec;30(12):3273-7. doi: 10.1007/s11274-014-1744-7. Epub 2014 Sep 26.
The Bacillus thuringiensis strain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea, cry30 Ga, cry50Ba and cry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30 kDa. In this study we used mass spectrometry to identify the 130 kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity against Culex quinquefasciatus.
苏云金芽孢杆菌菌株S2160 - 1先前已被鉴定为对蚊虫幼虫具有高毒性，并且是目前商业上用于控制这些昆虫的菌株的可行替代品。一种聚合酶链式反应（PCR）方法已确定该菌株中存在四个假定的杀虫毒素基因（cry30Ea、cry30Ga、cry50Ba和cry54Ba），但未鉴定出编码大小为140 kDa、130 kDa和30 kDa的三种主要晶体毒素蛋白的基因。在本研究中，我们使用质谱法将130 kDa毒素鉴定为一种罕见的Cry4毒素（Cry4Cb3）。编码该毒素的基因被克隆并表达，并且该毒素显示出对致倦库蚊具有杀蚊活性。
