Kamba Seiji, Seto Hirokazu, Kondo Takashi, Miura Yoshiko
Murata Manufacturing Co., Ltd.
Graduate School of Engineering, Kyushu University.
Anal Sci. 2017;33(9):1033-1039. doi: 10.2116/analsci.33.1033.
Biosensing of protein adsorption with metal mesh device (MMD) was investigated by computational calculations and experiments. Electromagnetic field computation was carried out with a single unit cell of MMD. Equivalent circuit model of MMD on the single unit cell was assumed, and the biosensing with MMD was analyzed in detail by computational calculation and experimental measurements. The dip frequency of MMD was shifted by adsorption of protein on MMD. The shift of dip frequency of MMD was proportional to the amount of protein adsorption. The sensitivity of MMD biosensing was dependent on the microstructure of MMD, and proportional to the square of the dip frequency. The refinement of MMD structure can improve the sensitivity of protein detection.
利用计算模拟和实验研究了金属网格装置（MMD）对蛋白质吸附的生物传感。使用MMD的单个单元胞进行了电磁场计算。假设了MMD在单个单元胞上的等效电路模型，并通过计算模拟和实验测量详细分析了MMD的生物传感。MMD的凹陷频率因蛋白质在MMD上的吸附而发生偏移。MMD凹陷频率的偏移与蛋白质吸附量成正比。MMD生物传感的灵敏度取决于MMD的微观结构，且与凹陷频率的平方成正比。MMD结构的优化可以提高蛋白质检测的灵敏度。
