Geng Yanfang, Ko Euna, Tran Van-Khue, Chung Woo Sung, Park Chan Ho, Kim Min Ki, Jin Ga Hyun, Seong Gi Hun
Department of Bionano Engineering, Hanyang University.
Anal Sci. 2017;33(9):993-998. doi: 10.2116/analsci.33.993.
In the present study, we developed an electrochemical sensor for highly sensitive detection of hydroxylamine using Au-Pt alloy nanoparticles. Au-Pt alloy nanoparticles were electrochemically deposited on a working electrode made of single-walled carbon nanotubes. The framework composition in the Au-Pt alloy nanoparticle was easily controlled by adjusting the Au:Pt composition ratio in the precursor solution. Morphological and chemical characterizations of the resulting Au-Pt alloy nanoparticles were performed using field emission scanning electron microscopy, X-ray diffraction, and energy dispersion X-ray spectroscopy. When the Au:Pt ratio in the precursor solution was 1:5, the ratio of Au:Pt atom in alloy nanoparticle was about 6:4. AuPt alloy nanoparticles were found to have the optimum synthetic ratio for hydroxylamine detection. The electrocatalytic performance of Au-Pt alloy nanoparticles in the presence of hydroxylamine was also characterized using cyclic voltammetry, differential pulse voltammetry, and chronoamperometry. In the chronoamperometric detection of hydroxylamine, the sensor exhibited a detection limit of 0.80 μM (S/N = 3) and a high sensitivity of 184 μA mM cm. Moreover, the amperometric response of the sensor in 1 mM hydroxylamine was stable for a long time (450 s). Long-term stability tests showed that the current responses to hydroxylamine were 96, 91 and 85% of the initial signal value after storage for 5, 10, and 20 days, respectively.
在本研究中，我们开发了一种用于高灵敏度检测羟胺的电化学传感器，该传感器使用金铂合金纳米颗粒。金铂合金纳米颗粒通过电化学方法沉积在由单壁碳纳米管制成的工作电极上。通过调节前驱体溶液中的金铂组成比，可以轻松控制金铂合金纳米颗粒的骨架组成。使用场发射扫描电子显微镜、X射线衍射和能量色散X射线光谱对所得金铂合金纳米颗粒进行形态和化学表征。当前驱体溶液中的金铂比为1:5时，合金纳米颗粒中的金铂原子比约为6:4。发现金铂合金纳米颗粒具有检测羟胺的最佳合成比例。还使用循环伏安法、差分脉冲伏安法和计时电流法对金铂合金纳米颗粒在羟胺存在下的电催化性能进行了表征。在羟胺的计时电流检测中，该传感器的检测限为$0.80\ \mu M$（信噪比 = 3），灵敏度高达$184\ \mu A\ mM^{-1}\ cm^{-2}$。此外，该传感器在$1\ mM$羟胺中的安培响应在很长时间内（450 s）保持稳定。长期稳定性测试表明，在储存5、10和20天后，对羟胺的电流响应分别为初始信号值的96%、91%和85%。
