Bijan Chemistry Laboratory, Mashhad, Khorasan, Iran.
Mycotoxin Res. 2018 Mar;34(1):15-20. doi: 10.1007/s12550-017-0294-x. Epub 2017 Sep 10.
A rapid, simple, and green vortex-assisted emulsification microextraction method based on solidification of floating organic drop was developed for the extraction and determination of ochratoxin A (OTA) with high-performance liquid chromatography. Some factors influencing the extraction efficiency of OTA such as the type and volume of extraction solvent, sample pH, salt concentration, vortex time, and sample volume were optimized. Under optimized conditions, the calibration curve exhibited linearity in the range of 50.0-500 ng L with a coefficient of determination higher than 0.999. The limit of detection was 15.0 ng L. The inter- and intra-assays relative standard deviations were in a range of 4.7-8.7%. The accuracy of the developed method was investigated through recovery experiments, and it was successfully used for the quantification of OTA in 40 samples of fruit juice.
建立了一种基于浮相固体化的快速、简单、绿色的涡旋辅助乳化微萃取方法，用于高效液相色谱法测定赭曲霉毒素 A（OTA）。优化了萃取 OTA 的一些因素，如萃取溶剂的类型和体积、样品 pH 值、盐浓度、涡旋时间和样品体积。在优化条件下，OTA 的校准曲线在 50.0-500ng/L 范围内呈线性，相关系数大于 0.999。检出限为 15.0ng/L。日内和日间相对标准偏差在 4.7-8.7%范围内。通过回收实验研究了该方法的准确性，并成功用于 40 份果汁样品中 OTA 的定量分析。
