Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, P. R. China.
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, P. R. China.
Electrophoresis. 2018 Jan;39(2):363-369. doi: 10.1002/elps.201700320. Epub 2017 Sep 26.
Pillar[n]arenes have achieved much interest in material chemistry and supramolecular chemistry due to unusual pillar shape structure and high selectivity toward guest. However, pillar[n]arenes have not yet been applied in capillary electrochromatography. This work at first time reports that carboxylatopillar[5]arene is used as a stationary phase in open-tubular capillary electrochromatography. Carboxylatopillar[5]arene not only possess the advantages of pillar[n]arenes but also provide free carboxy groups for immobilizing on the inner wall of capillary column via covalent bonding. The characterization of SEM and FT-IR indicated that carboxylatopillar[5]arene was successfully grafted on the inner wall of capillary. The baseline separation of model analytes including neutral, basic, and acidic compounds, nonsteroidal anti-inflammatory drugs and dansyl-amino acids have been achieved thanks to the electron-rich cavity of carboxylatopillar[5]arene and hydrophobic interactions between the analytes and stationary phase. The intraday, interday, and column-to-column precisions (RSDs) of retention time and peak area for the neutral analytes were all less than 3.34 and 9.65%, respectively. This work indicates that pillar[n]arenes have great potential in capillary electrochromatography as novel stationary phase.
[柱芳烃]因其独特的柱状结构和对客体的高选择性，在材料化学和超分子化学领域引起了广泛关注。然而，[柱芳烃]尚未应用于毛细管电色谱。本工作首次报道了羧基化[5]柱芳烃作为固定相在开管毛细管电色谱中的应用。羧基化[5]柱芳烃不仅具有[柱芳烃]的优点，而且还提供了自由的羧基，可通过共价键合固定在毛细管柱的内壁上。SEM 和 FT-IR 的表征表明，羧基化[5]柱芳烃已成功接枝到毛细管内壁上。由于羧基化[5]柱芳烃富电子空腔和分析物与固定相之间的疏水相互作用，实现了包括中性、碱性和酸性化合物、非甾体抗炎药和丹磺酰基氨基酸在内的模型分析物的基线分离。中性分析物的保留时间和峰面积的日内、日间和柱间精密度（RSD）均小于 3.34%和 9.65%。这项工作表明，[柱芳烃]作为新型固定相在毛细管电色谱中有很大的应用潜力。
