Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China.
Talanta. 2018 Feb 1;178:650-655. doi: 10.1016/j.talanta.2017.10.004. Epub 2017 Oct 3.
Hypercrosslinked polymers (HCPs) are currently receiving great attention due to their unique characteristics and potential uses in diverse areas. However, the field of HCPs for open-tubular capillary electrochromatography (OT-CEC) separations has not been explored. Here, a knitted aromatic polymer (KAP) was in-situ grown on the inner wall of the capillary column for OT-CEC for the first time. The silylating reagent containing phenyl was served as monomer, immobilized on the inner wall of the capillary column, and then KAPs-modified capillary column was prepared through in-situ hypercrosslinking reaction. The surface structure and morphology of KAPs-modified capillary column was characterized by Fourier transform infrared spectra (FT-IR) and scanning electron microscopy (SEM). The prepared capillary columns showed good separation performance for neutral compounds, small biomolecules, such as nucleosides, amino acids, small peptides, and non-steroidal anti-inflammatory drugs, sulfa drugs. In addition, the KAPs-modified capillaries showed good reproducibility, with relative standard deviations for intra-day, inter-day and column-to-column runs less than 1.59%, 2.55%, and 5.19% respectively. The strategy of in-situ immobilization of KAPs provides a new approach for the application of the material in the analytical fields.
超交联聚合物（HCPs）因其独特的特性和在各个领域的潜在用途而受到广泛关注。然而，HCPs 在开管毛细管电色谱（OT-CEC）分离中的应用尚未得到探索。本文首次在毛细管柱内壁原位生长编织芳香聚合物（KAP）用于 OT-CEC。以含苯基的硅烷化试剂作为单体，固定在毛细管柱内壁上，然后通过原位超交联反应制备 KAPs 修饰的毛细管柱。通过傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对 KAPs 修饰的毛细管柱的表面结构和形态进行了表征。所制备的毛细管柱对中性化合物、小生物分子（如核苷、氨基酸、小肽和非甾体抗炎药、磺胺类药物）具有良好的分离性能。此外，KAPs 修饰的毛细管柱具有良好的重现性，日内、日间和柱间运行的相对标准偏差分别小于 1.59%、2.55%和 5.19%。KAPs 的原位固定策略为该材料在分析领域的应用提供了一种新方法。
