Ma Huifen, Bai Yun, Li Jin, Chang Yan-Xu
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
Key Laboratory of Formula of Traditional Chinese Medicine (Tianjin University of Traditional Chinese Medicine), Ministry of Education, Tianjin, P. R. China.
Electrophoresis. 2018 Jan;39(1):260-274. doi: 10.1002/elps.201700239. Epub 2017 Oct 4.
Bioactive ingredients of natural products can protect human body from harm, as well as prevent and treat disease. Screening bioactive compounds from natural products is attracting particular attention. It is a great challenge to separate and detect the active compounds from complex matrix natural products. CE plays a vital role in screening active compounds because of its unique features such as high-efficiency separation, short-analysis time, minimal sample consumption, and ease to realize automatization etc. Additionally, CE has been developed various modes owing to its abundant advantages in analysis and separation of compounds. The purpose of this work is to review previous developments and applications of CE in screening bioactive compounds derived from natural products from 2007 to 2017. This review does not only summarize the traditional methods of detecting active ingredients but also briefly introduces some novel assays. The trends in the application of CE in active compounds screening are addressed in the article.
天然产物的生物活性成分可以保护人体免受伤害，以及预防和治疗疾病。从天然产物中筛选生物活性化合物正受到特别关注。从复杂基质的天然产物中分离和检测活性化合物是一项巨大的挑战。毛细管电泳（CE）因其具有高效分离、分析时间短、样品消耗少以及易于实现自动化等独特特性，在筛选活性化合物方面发挥着至关重要的作用。此外，由于CE在化合物分析和分离方面具有诸多优势，已开发出多种模式。这项工作的目的是回顾2007年至2017年期间CE在筛选天然产物衍生的生物活性化合物方面的先前发展和应用。本综述不仅总结了检测活性成分的传统方法，还简要介绍了一些新颖的分析方法。本文还探讨了CE在活性化合物筛选中的应用趋势。
