Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
Key Laboratory of Formula of Traditional Chinese Medicine (Tianjin University of Traditional Chinese Medicine), Ministry of Education, Tianjin, 300193, China.
Sci Rep. 2018 Apr 3;8(1):5441. doi: 10.1038/s41598-018-23748-x.
A novel method of on-line 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)-Capillary Electrophoresis-Diode Array Detector (on-line ABTS-CE-DAD) was developed to screen the major antioxidants from complex herbal medicines. ABTS, one of well-known oxygen free radicals was firstly integrated into the capillary. For simultaneously detecting and separating ABTS and chemical components of herb medicines, some conditions were optimized. The on-line ABTS-CE-DAD method has successfully been used to screen the main antioxidants from Shuxuening injection (SI), an herbal medicines injection. Under the optimum conditions, nine ingredients of SI including clitorin, rutin, isoquercitrin, Quercetin-3-O-D-glucosyl]-(1-2)-L-rhamnoside, kaempferol-3-O-rutinoside, kaempferol-7-O-β-D-glucopyranoside, apigenin-7-O-Glucoside, quercetin-3-O-[2-O-(6-O-p-hydroxyl-E-coumaroyl)-D-glucosyl]-(1-2)-L-rhamnoside, 3-O-{2-O-[6-O-(p-hydroxyl-E-coumaroyl)-glucosyl]}-(1-2) rhamnosyl kaempfero were separated and identified as the major antioxidants. There is a linear relationship between the total amount of major antioxidants and total antioxidative activity of SI with a linear correlation coefficient of 0.9456. All the Relative standard deviations of recovery, precision and stability were below 7.5%. Based on these results, these nine ingredients could be selected as combinatorial markers to evaluate quality control of SI. It was concluded that on-line ABTS-CE-DAD method was a simple, reliable and powerful tool to screen and quantify active ingredients for evaluating quality of herbal medicines.
建立了一种新的在线 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）-毛细管电泳-二极管阵列检测（在线 ABTS-CE-DAD）方法，用于筛选复杂中草药中的主要抗氧化剂。ABTS 是一种著名的氧自由基，首先被整合到毛细管中。为了同时检测和分离 ABTS 和草药化学组分，对一些条件进行了优化。该在线 ABTS-CE-DAD 方法已成功用于筛选舒血宁注射液（SI）中的主要抗氧化剂，SI 是一种中草药注射液。在最佳条件下，从 SI 中分离并鉴定出九种成分，包括 Clitorin、芦丁、异槲皮苷、槲皮素-3-O-D-葡萄糖基]-(1-2)-L-鼠李糖苷、山奈酚-3-O-芸香糖苷、山奈酚-7-O-β-D-吡喃葡萄糖苷、芹菜素-7-O-葡萄糖苷、槲皮素-3-O-[2-O-(6-O-对羟基肉桂酰基)-D-葡萄糖基]-(1-2)-L-鼠李糖苷、3-O-{2-O-[6-O-(对羟基肉桂酰基)-葡萄糖基]}-(1-2)鼠李糖苷山奈酚。SI 中的主要抗氧化剂总量与总抗氧化活性之间存在线性关系，线性相关系数为 0.9456。回收率、精密度和稳定性的相对标准偏差均低于 7.5%。基于这些结果，这九种成分可被选为组合标志物，用于评价 SI 的质量控制。结论：在线 ABTS-CE-DAD 方法是一种简单、可靠、强大的工具，可用于筛选和定量评估中草药质量的活性成分。
