基于 CRISPR-Cas9 的光激活转录系统诱导神经元分化。
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
机构信息
Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11.
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
我们改进的基于 CRISPR-Cas9 的光激活转录系统，CPTS2.0 和 Split-CPTS2.0，可在各种人类细胞系中实现内源靶基因的高光诱导激活。我们通过 CPTS2.0 实现了靶基因的可逆激活，并通过 Split-CPTS2.0 上调 NEUROD1 诱导诱导多能干细胞（iPSC）中的神经元分化。
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