Molecular Microbiology Research and Applied Laboratory, Public Health Agency of Turkey, Ankara, Turkey.
Department of Medical Microbiology, Faculty of Medicine Yildirim Beyazit University, Ankara, Turkey.
J Med Virol. 2018 Feb;90(2):229-238. doi: 10.1002/jmv.24945. Epub 2017 Oct 6.
Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2-year sentinel surveillance study. A total of 1639 rotavirus antigen-positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT-PCR) with consensus primers for the VP7 and VP4 genes, followed by semi-nested type-specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0-12 months age group, followed by children in the 13-24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014-2016.
人轮状病毒 A（RVA）是全球 5 岁以下儿童水样腹泻的主要病因。本研究旨在调查在为期 2 年的监测研究中，土耳其流行的 RVA 基因型的流行率和多样性。从因急性胃肠炎住院的 5 岁以下儿童中收集了 1639 份轮状病毒抗原阳性粪便样本。使用针对 VP7 和 VP4 基因的共识引物通过逆转录聚合酶链反应（RT-PCR）确定轮状病毒 G 和 P 基因型，然后进行半巢式型特异性多重 PCR。在 1396 个（85.3%）测试样本中检测到轮状病毒 RNA。0-12 个月年龄组的儿童检测到的最高检出率（38.2%），其次是 13-24 个月年龄组（36.2%）。最常见的基因型是 G1P[8]（24.6%），其次是 G3P[8]（19.6%）、G9P[8]（12.2%）、G2P[4]（9.5%）、G2P[8]（6.5%）和 G4P[8]（4.8%）。罕见和混合基因型的比例分别为 21.5%和 1.14%。观察到的基因型数量众多，包括常见、罕见和混合类型，表明 RVA 株在土耳其的流行具有高度异质性。本研究还显示，常见基因型的流行率出现明显波动，G3 和 G1 的流行率增加，G9 和 G2 的流行率降低，从 2014-2016 年。
