Department of Molecular Epidemiology of Viral Infections, I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Nizhny Novgorod, Russia.
Braz J Microbiol. 2023 Dec;54(4):2867-2877. doi: 10.1007/s42770-023-01155-3. Epub 2023 Oct 28.
Since 2013, there has been an increase in reports of the spread of a double intergroup reassortant strain of rotavirus type A (RVA) with the genotype G3P[8] and other genes belonging to the second genogroup I2-R2-C2-M2-A2-N2-T2-E2-H2. In our study, we provide a molecular genetic characterization of rotaviruses with genotype G3P[8]-I2 isolated in Nizhny Novgorod. In our study, we used RT-PCR, Sanger sequencing, RNA-PAGE methods. Phylogenetic and phylodynamic analysis were performed using the Bayesian approach. According to our study, there was a significant increase in the proportion of G3P[8] from 15% during the period of 2020-2021 to 53% during the period of 2021-2022 in Nizhny Novgorod, Russia. Phylogenetic analysis based on the VP4 gene revealed that DS-1-like RVAs isolated in Nizhny Novgorod belong to different clusters of the P[8]-3.1 lineage, with a level of variation ranging from 1.1% to 1.3%. Based on the VP6 gene, the equine-like RVAs identified by us carry genetic variants belonging to three distinct clusters of the lineage I2-V, with a variation level ranging from 2.0% to 4.5%. These data indicate the genotypic diversity of circulating DS-1-like G3 RVAs. Phylogenetic analysis of the VP7 gene allowed us to assign the isolates identified in our study to the G3-1 lineage. We estimated that the circulation of the most recent common ancestor of the spreading strains dates back to 2002. Additionally, we determined the typical level of mutations in the VP7 gene, which amounted to 2.14*10-3 substitutions/per site/per year.
自 2013 年以来，报告显示 A 型轮状病毒（RVA）的双重群内重组株（基因型为 G3P[8]和其他属于第二基因组群 I2-R2-C2-M2-A2-N2-T2-E2-H2 的基因）传播有所增加。在我们的研究中，我们提供了在俄罗斯下诺夫哥罗德分离的基因型为 G3P[8]-I2 的轮状病毒的分子遗传特征。在我们的研究中，我们使用了 RT-PCR、Sanger 测序、RNA-PAGE 方法。使用贝叶斯方法进行了系统发育和系统动力学分析。根据我们的研究，在俄罗斯下诺夫哥罗德，G3P[8]的比例从 2020-2021 年期间的 15%显著增加到 2021-2022 年期间的 53%。基于 VP4 基因的系统发育分析表明，在下诺夫哥罗德分离的 DS-1 样 RVAs 属于 P[8]-3.1 谱系的不同聚类，变异程度在 1.1%-1.3%之间。基于 VP6 基因，我们鉴定的马源样 RVAs 携带属于 I2-V 谱系三个不同聚类的遗传变异，变异程度在 2.0%-4.5%之间。这些数据表明循环的 DS-1 样 G3 RVAs 的基因型多样性。VP7 基因的系统发育分析使我们能够将本研究中鉴定的分离株归入 G3-1 谱系。我们估计传播株最近共同祖先的流行时间可以追溯到 2002 年。此外，我们确定了 VP7 基因中典型的突变水平，为 2.14*10-3 个替换/位点/年。
