Balestrino Fabrizio, Puggioli Arianna, Carrieri Marco, Bouyer Jérémy, Bellini Romeo
Insect Pest Control Laboratory, NAFA Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, FAO/IAEA Agriculture and Biotechnology Laboratories, Seibersdorf, Austria.
Medical and Veterinary Entomology Department, Centro Agricoltura Ambiente CAA "G. Nicoli", Crevalcore, Italy.
PLoS Negl Trop Dis. 2017 Sep 11;11(9):e0005881. doi: 10.1371/journal.pntd.0005881. eCollection 2017 Sep.
The capacity of the released sterile males to survive, disperse, compete with wild males and inseminate wild females is an essential prerequisite to be evaluated in any area-wide integrated pest management (AW-IPM) programs including a sterile insect release method. Adequate quality control tests supported by standardized procedures need to be developed to measure these parameters and to identify and correct potential inappropriate rearing or handling methods affecting the overall male quality. In this study, we report results on the creation and validation of the first quality control devices designed to infer the survival and mating capacity of radio-sterilized Aedes albopictus males through the observation of their flight capacity under restricted conditions (flight organ device) and after stress treatment (aspirator device). Results obtained consistently indicate comparable flight capacity and quality parameters between untreated and 35 Gy irradiated males while a negative impact was observed with higher radiation doses at all observation time performed. The male flight capacity registered with the proposed quality control devices can be successfully employed, with different predictive capacities and response time, to infer the adult male quality. These simple and cost-effective tools provide a valuable method to detect and amend potentially sub-standard procedures in the sterile male production line and hence contribute to maintaining optimal quality and field performance of the mosquitoes being released.
在任何包括不育昆虫释放方法的区域综合虫害管理（AW-IPM）计划中，评估释放的不育雄虫的生存、扩散、与野生雄虫竞争以及使野生雌虫受精的能力是一项基本前提条件。需要制定由标准化程序支持的充分质量控制测试，以测量这些参数，并识别和纠正影响雄虫整体质量的潜在不适当饲养或处理方法。在本研究中，我们报告了首个质量控制装置的创建和验证结果，该装置旨在通过观察白纹伊蚊雄虫在受限条件下（飞行器官装置）和应激处理后（吸气装置）的飞行能力，推断经辐射绝育的雄虫的生存和交配能力。在所有观察时间点，所获得的结果一致表明，未处理雄虫和35 Gy辐照雄虫之间的飞行能力和质量参数相当，而在更高辐射剂量下观察到了负面影响。使用所提议的质量控制装置记录的雄虫飞行能力，可成功用于以不同的预测能力和响应时间推断成年雄虫的质量。这些简单且经济高效的工具提供了一种有价值的方法，用于检测和修正不育雄虫生产线中潜在的不合格程序，从而有助于维持所释放蚊子的最佳质量和田间性能。
