Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden.
Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden.
Bioresour Technol. 2017 Dec;245(Pt A):145-151. doi: 10.1016/j.biortech.2017.08.125. Epub 2017 Aug 24.
The use of hot-water (100°C) from the 1st generation ethanol plants for mild-temperature lignocellulose pretreatment can possibly cut down the operational (energy) cost of 2nd generation ethanol process, in an integrated model. Dilute-sulfuric and -phosphoric acid pretreatment at 100°C was carried out for wheat bran and whole-stillage fibers. Pretreatment time and acid type influenced the release of sugars from wheat bran, while acid-concentration was found significant for whole-stillage fibers. Pretreatment led up-to 300% improvement in the glucose yield compared to only-enzymatically treated substrates. The pretreated substrates were 191-344% and 115-300% richer in lignin and glucan, respectively. Fermentation using Neurospora intermedia, showed 81% and 91% ethanol yields from wheat bran and stillage-fibers, respectively. Sawdust proved to be a highly recalcitrant substrate for mild-temperature pretreatment with only 22% glucose yield. Both wheat bran and whole-stillage are potential substrates for pretreatment using waste heat from the 1st generation process for 2nd generation ethanol.
第一代乙醇工厂使用的热水（100°C）可用于温和温度木质纤维素预处理，在集成模型中可能降低第二代乙醇工艺的运行（能源）成本。在 100°C 下进行稀硫酸和稀磷酸预处理，用于麦麸和全糟纤维。预处理时间和酸类型影响麦麸中糖的释放，而酸浓度对全糟纤维的影响较大。与仅酶处理的底物相比，预处理可将葡萄糖产率提高 300%。预处理后的底物的木质素和葡聚糖含量分别增加了 191-344%和 115-300%。利用粗糙脉孢菌进行发酵，从麦麸和酒糟纤维中分别获得 81%和 91%的乙醇产率。木屑在温和温度预处理中表现出高度的抗性，葡萄糖产率仅为 22%。麦麸和全糟都是利用第一代工艺的废热进行第二代乙醇预处理的潜在底物。
