2016年至2017年冬季，五种不同的H5N6高致病性甲型禽流感病毒重配体影响了日本。

Five distinct reassortants of H5N6 highly pathogenic avian influenza A viruses affected Japan during the winter of 2016-2017.

作者信息

Takemae Nobuhiro, Tsunekuni Ryota, Sharshov Kirill, Tanikawa Taichiro, Uchida Yuko, Ito Hiroshi, Soda Kosuke, Usui Tatsufumi, Sobolev Ivan, Shestopalov Alexander, Yamaguchi Tsuyoshi, Mine Junki, Ito Toshihiro, Saito Takehiko

机构信息

Division of Transboundary Animal Disease, National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Thailand-Japan Zoonotic Diseases Collaboration Center, Kasetklang, Chatuchak, Bangkok 10900, Thailand.

Research Institute of Experimental and Clinical Medicine, 2, Timakova street, Novosibirsk 630117, Russia.

出版信息

Virology. 2017 Dec;512:8-20. doi: 10.1016/j.virol.2017.08.035. Epub 2017 Sep 9.

DOI:10.1016/j.virol.2017.08.035

PMID:28892736

Abstract

To elucidate the evolutionary pathway, we sequenced the entire genomes of 89 H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated in Japan during winter 2016-2017 and 117 AIV/HPAIVs isolated in Japan and Russia. Phylogenetic analysis showed that at least 5 distinct genotypes of H5N6 HPAIVs affected poultry and wild birds during that period. Japanese H5N6 isolates shared a common genetic ancestor in 6 of 8 genomic segments, and the PA and NS genes demonstrated 4 and 2 genetic origins, respectively. Six gene segments originated from a putative ancestral clade 2.3.4.4 H5N6 virus that was a possible genetic reassortant among Chinese clade 2.3.4.4 H5N6 HPAIVs. In addition, 2 NS clusters and a PA cluster in Japanese H5N6 HPAIVs originated from Chinese HPAIVs, whereas 3 distinct AIV-derived PA clusters were evident. These results suggest that migratory birds were important in the spread and genetic diversification of clade 2.3.4.4 H5 HPAIVs.

摘要

为阐明进化途径，我们对2016 - 2017年冬季在日本分离的89株H5N6高致病性禽流感病毒（HPAIV）以及在日本和俄罗斯分离的117株禽流感病毒/高致病性禽流感病毒进行了全基因组测序。系统发育分析表明，在此期间至少有5种不同基因型的H5N6 HPAIV感染了家禽和野鸟。日本的H5N6分离株在8个基因组片段中的6个上有一个共同的遗传祖先，而PA和NS基因分别显示出4个和2个遗传起源。6个基因片段起源于一个假定的祖先2.3.4.4 H5N6病毒分支，该病毒可能是中国2.3.4.4 H5N6 HPAIV之间的基因重配体。此外，日本H5N6 HPAIV中的2个NS簇和1个PA簇起源于中国的HPAIV，而明显存在3个不同的源自禽流感病毒的PA簇。这些结果表明候鸟在2.3.4.4 H5 HPAIV的传播和基因多样化中起重要作用。