Naregal Govindanagouda V, Devaranavadagi Basavaraj B, Patil Satish G, Aski Basavaraj S
Tutor, Department of Biochemistry, BLDE University's Sri B M Patil Medical College, Hospital and Research Centre, Vijayapura, Karnataka, India.
Professor, Department of Biochemistry, BLDE University's Sri B M Patil Medical College, Hospital and Research Centre, Vijayapura, Karnataka, India.
J Clin Diagn Res. 2017 Jul;11(7):BC09-BC12. doi: 10.7860/JCDR/2017/27931.10252. Epub 2017 Jul 1.
Hypertension is becoming an important medical and public health problem all over the world and is the most common disorder of ageing. There is a growing evidence of involvement of vascular oxidative stress in the development of hypertension from animal studies. However, studies on humans with hypertension, particularly in elderly are least and data remained controversial. Moreover, studies in elderly people with hypertension are scarce.
To investigate the possible role of oxidative stress and antioxidant defense in the pathogenesis of hypertension in elderly.
A cross-sectional study was conducted on elderly males (n=60) with newly diagnosed hypertension and with normal blood pressure. Oxidative stress and antioxidant status were evaluated by assessing the following parameters: plasma Malondialdehyde (MDA), and antioxidants: Superoxide Dismutase (SOD) activity, reduced Glutathione (GSH), and vitamin C levels; and total Nitric Oxide concentration in plasma (NOx). Difference between groups was determined by using unpaired t-test/Mann-Whitney U test. Bivariate correlation and multiple regression analysis were used to determine the relationship between variables.
A significant rise in plasma MDA (p-value=0.013) and lower levels of endogenous antioxidants: SOD (p-value≤0.001) and GSH (p-value≤0.001) were observed in elderly individuals with hypertension when compared to healthy controls. Though not significant, there was a mean decrease in plasma NOx in hypertensive subjects than normotensive ones. While vitamin C showed no significant difference between two groups. Decrease in GSH (β=-0.398; p-value=0.001) and SOD (β=-0.423; p-value≤0.001) were the significant determinants of hypertension in elderly individuals.
Above findings indicate that elevation in oxidative stress and decrease in endogenous antioxidant level may be involved in the pathogenesis of hypertension. However, it remains unclear whether oxidative stress causes or augments hypertension.
高血压正在成为全球一个重要的医学和公共卫生问题，并且是衰老过程中最常见的病症。动物研究越来越多地证明血管氧化应激参与高血压的发展。然而，关于高血压患者，尤其是老年患者的研究极少，数据仍存在争议。此外，针对老年高血压患者的研究也很匮乏。
探讨氧化应激和抗氧化防御在老年高血压发病机制中的可能作用。
对60名新诊断的老年男性高血压患者和血压正常者进行了一项横断面研究。通过评估以下参数来评价氧化应激和抗氧化状态：血浆丙二醛（MDA）以及抗氧化剂：超氧化物歧化酶（SOD）活性、还原型谷胱甘肽（GSH）和维生素C水平；以及血浆总一氧化氮浓度（NOx）。采用非配对t检验/曼-惠特尼U检验确定组间差异。使用双变量相关性和多元回归分析来确定变量之间的关系。
与健康对照组相比，老年高血压患者血浆MDA显著升高（p值 = 0.013），内源性抗氧化剂SOD（p值≤0.001）和GSH（p值≤0.001）水平降低。虽然不显著，但高血压患者的血浆NOx均值低于血压正常者。而两组间维生素C无显著差异。GSH降低（β = -0.398；p值 = 0.001）和SOD降低（β = -0.423；p值≤0.001）是老年个体高血压的重要决定因素。
上述研究结果表明，氧化应激升高和内源性抗氧化剂水平降低可能参与高血压的发病机制。然而，氧化应激是导致还是加剧高血压仍不清楚。
