Department of Biochemistry, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland.
Redox Rep. 2011;16(2):71-7. doi: 10.1179/174329211X13002357050897.
Oxidative stress has been reported to increase with aging. Oxidative stress is also associated with hypertension, and antioxidant treatment has been shown to enhance antioxidant defense system. We therefore aimed to analyze the relationship between aging and some markers of oxidative stress in elderly patients with essential hypertension compared with healthy controls.
Blood was collected from 18 patients with essential hypertension and 21 age- and sex-matched healthy controls aged over 65. Patients were on their usual medications while participating in the study. Oxidative stress parameters were investigated by measuring the concentration of glutathione (GSH) in whole blood and activities of glutathione peroxidase (GPx-1), glutathione reductase (GR), catalase (CAT), and Cu-Zn superoxide dismutase (CuZn SOD, SOD-1) in erythrocytes. GSH, GPx-1, GR, CAT, and CuZn SOD correlations with age were expressed as Pearson product-moment correlation coefficient r. Independent-samples T test was used to compare mean values of parameters between groups.
(1) Among all parameters analyzed herein, the activity of SOD-1 showed the most explicit decrease in relation to age, both in healthy controls and hypertensive subjects with r values of -0.54 (P = 0.05) and -0.68 (P < 0.01), respectively. (2) Age-related changes in parameters of oxidative stress did not differ significantly between groups. (3) Mean activity of SOD-1 was significantly higher (P < 0.05) in elderly hypertensives (2341.7 ± 213.71 U/g Hb) when compared with healthy controls (2199.7 ± 213.66 U/g Hb). (4) Mean GSH level was significantly higher (P < 0.01) in patients (3.1 ± 0.29 mmol/l) than in controls (2.8 ± 0.37 mmol/l). (5) Increased level of GSH in hypertension was followed by significantly (P < 0.01) higher activity of GR in this group when compared with controls (83.4 ± 15.25 and 64.1 ± 9.40 U/g Hb, respectively).
(1) The antioxidant barrier changes in elderly subjects with senescence. (2) CuZn SOD activity is negatively correlated with age and this association is not altered by factors that modulate the enzyme activity, such as hypertension and antihypertensive treatment. (3) Significantly higher concentration of GSH and significantly higher GR activity in patients may suggest a significant role of GSH metabolism in the pathogenesis of hypertension, as well as its contribution to the effect of antihypertensive treatment.
氧化应激据报道会随着年龄的增长而增加。氧化应激也与高血压有关，抗氧化治疗已被证明能增强抗氧化防御系统。因此，我们旨在分析与健康对照组相比，老年原发性高血压患者的衰老与一些氧化应激标志物之间的关系。
从 18 名原发性高血压患者和 21 名年龄和性别匹配的 65 岁以上的健康对照者中采集血液。患者在参与研究时正在服用常规药物。通过测量全血中谷胱甘肽 (GSH) 的浓度以及红细胞中谷胱甘肽过氧化物酶 (GPx-1)、谷胱甘肽还原酶 (GR)、过氧化氢酶 (CAT) 和铜锌超氧化物歧化酶 (CuZn SOD，SOD-1) 的活性来研究氧化应激参数。GSH、GPx-1、GR、CAT 和 CuZn SOD 与年龄的相关性用 Pearson 积矩相关系数 r 表示。使用独立样本 t 检验比较组间参数的平均值。
(1)在所分析的所有参数中，SOD-1 的活性与年龄的相关性最为明显，健康对照组和高血压组的 r 值分别为-0.54（P=0.05）和-0.68（P<0.01）。(2)氧化应激参数的年龄相关性变化在两组之间无显著差异。(3)与健康对照组（2199.7±213.66 U/g Hb）相比，老年高血压患者（2341.7±213.71 U/g Hb）的 SOD-1 活性明显更高（P<0.05）。(4)患者的 GSH 水平明显高于对照组（3.1±0.29 mmol/l 比 2.8±0.37 mmol/l，P<0.01）。(5)与对照组（83.4±15.25 和 64.1±9.40 U/g Hb）相比，高血压患者中 GSH 水平升高与 GR 活性显著升高（P<0.01）相关。
(1) 老年患者抗氧化屏障随衰老而变化。(2) CuZn SOD 活性与年龄呈负相关，这种相关性不受调节酶活性的因素（如高血压和降压治疗）的改变。(3) 患者 GSH 浓度显著升高和 GR 活性显著升高可能表明 GSH 代谢在高血压发病机制中具有重要作用，以及其对降压治疗效果的贡献。
