Bhoge Amit, Khandeparkar Siddhi Gaurish Sinai, Joshi Avinash R, Gogate Bageshri, Kulkarni Maithili Mandar, Bhayekar Pallavi
Postgraduate Student, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India.
Associate Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India.
J Clin Diagn Res. 2017 Jul;11(7):EC12-EC16. doi: 10.7860/JCDR/2017/26537.10230. Epub 2017 Jul 1.
Immunohistochemical (IHC) markers of mucin family are associated with various Gallbladder Lesions (GBLs).
To study the distribution of GBL with respect to age and sex as well as to analyse the IHC profile of MUC1 and MUC5AC in GBLs and attempt correlation with clinical and histopathological findings.
The present study was conducted over a period of six years. A technique of manual tissue array was employed for cases subjected to IHC using MUC1 and MUC5AC. Results were statistically analysed using software program "The Primer of Biostatistics 5.0".
A total of 629 GBL were encountered. Out of 605 of non-neoplastic lesions, 32 (5.29%) expressed MUC1 while 515 (85.12%) cases expressed MUC5AC. Out of 24 cases of neoplastic GBL, 20 cases (83.33%) showed positivity for MUC1 and 9 cases (37.5%) were positive for MUC5AC. The rate of MUC1 expression was significantly higher in Gall Bladder Cancer (GBC) {18GB carcinoma (ca) +3 Carcinoma In Situ (CIS)} (85.71%) than chronic cholecystitis (4.71%). The positive rate of MUC5AC expression was significantly lower in GBC (28.57%) than chronic cholecystitis (87.19%). The percentage of cases showing MUC1 expression increased as the severity of disease progressed from hyperplasia to CIS. The percentage of cases showing MUC5AC expression decreased as the severity of disease progressed from hyperplasia to CIS.
In this study, 96.18% cases were non neoplastic GBL of which chronic cholecystitis (87.77%) was predominant. 3.81% of the GBL constituted for neoplastic lesions of which 75% were GBC. MUC1 showed higher rates of expression in neoplastic GBL. MUC5AC showed higher rates of expression in non neoplastic GBL. Expression of MUC1 and MUC5AC might be closely related to pathogenesis of neoplastic and non neoplastic GBL.
黏蛋白家族的免疫组织化学（IHC）标志物与各种胆囊病变（GBL）相关。
研究GBL在年龄和性别方面的分布情况，并分析GBL中MUC1和MUC5AC的免疫组化特征，并尝试与临床和组织病理学结果进行相关性分析。
本研究历时六年。采用手工组织芯片技术对使用MUC1和MUC5AC进行免疫组化的病例进行检测。使用软件程序“The Primer of Biostatistics 5.0”对结果进行统计学分析。
共遇到629例GBL。在605例非肿瘤性病变中，32例（5.29%）表达MUC1，而515例（85.12%）病例表达MUC5AC。在24例肿瘤性GBL中，20例（83.33%）MUC1呈阳性，9例（37.5%）MUC5AC呈阳性。胆囊癌（GBC）{18例胆囊癌（ca）+3例原位癌（CIS）}中MUC1的表达率（85.71%）显著高于慢性胆囊炎（4.71%）。GBC中MUC5AC的阳性表达率（28.57%）显著低于慢性胆囊炎（87.19%）。随着疾病严重程度从增生发展到原位癌，MUC1表达的病例百分比增加。随着疾病严重程度从增生发展到原位癌，MUC5AC表达的病例百分比降低。
在本研究中，96.18%的病例为非肿瘤性GBL，其中慢性胆囊炎（87.77%）占主导。3.81%的GBL为肿瘤性病变，其中75%为GBC。MUC1在肿瘤性GBL中显示出较高的表达率。MUC5AC在非肿瘤性GBL中显示出较高的表达率。MUC1和MUC5AC的表达可能与肿瘤性和非肿瘤性GBL的发病机制密切相关。
