Dhande Priti Pravin, Gupta Amit O, Jain Sourav, Dawane Jayshree Shriram
Associate Professor, Department of Pharmacology, Bharati Vidyapeeth Deemed University Medical College, Pune, Maharashtra, India.
Postgraduate Student, Department of Pharmacology, Bharati Vidyapeeth Deemed University Medical College, Pune, Maharashtra, India.
J Clin Diagn Res. 2017 Jul;11(7):FF01-FF04. doi: 10.7860/JCDR/2017/25081.10257. Epub 2017 Jul 1.
The incidence of arthritis is quite high and there is a need for the search of natural products to halt the progression of disease or provide symptomatic relief without significant adverse effects.
This study aimed at evaluating the anti-inflammatory and analgesic activities of topical in an animal model of chronic inflammation.
Albino rats of either sex were divided into five groups of six rats each (Group I - Control, Group II -Gel base, Group III - paste, Group IV - gel, Group V- Diclofenac gel). Chronic inflammation was induced on day 0 by injecting 0.1 ml Complete Freund's Adjuvant (CFA) in sub-plantar tissue of left hind paw of the rats. Topical treatment was started from day 12 till day 28. Body weight and paw volume (Plethysmometer) were assessed on day 0, 12 and 28. Pain assessment was done using Randall and Selitto paw withdrawal method. Data was analysed using GraphPad Prism version 5. Unpaired students t-test and ANOVA followed by Tukey's test was used for comparison among groups.
Only topical gel significantly reduced the body weight (p=0.02) due to reduction in inflammatory oedema of the left limb. gel also showed significant reduction (p=0.03) in paw volume of rats compared to the other groups. There was significant reduction in pain threshold (gm/sec) due to chronic inflammation, with all the study drugs (p<0.05) but with gel, this reduction was less (p<0.001).
Gel showed significant anti-inflammatory and mild analgesic activity on topical application in rat model of chronic inflammation.
关节炎的发病率相当高，因此需要寻找天然产物来阻止疾病进展或提供症状缓解且无明显不良反应。
本研究旨在评估[具体药物名称]外用制剂在慢性炎症动物模型中的抗炎和镇痛活性。
将雌雄白化大鼠分为五组，每组六只（第一组 - 对照组，第二组 - 凝胶基质组，第三组 - 糊剂组，第四组 - 凝胶组，第五组 - 双氯芬酸凝胶组）。在第0天，通过在大鼠左后爪足底组织注射0.1 ml完全弗氏佐剂（CFA）诱导慢性炎症。从第12天开始至第28天进行局部治疗。在第0、12和28天评估体重和爪体积（体积描记器）。使用兰德尔和塞利托爪撤离法进行疼痛评估。数据使用GraphPad Prism 5版本进行分析。采用非配对学生t检验和方差分析，随后进行Tukey检验用于组间比较。
仅[具体药物名称]凝胶由于左肢炎症性水肿减轻而显著降低了体重（p = 0.02）。与其他组相比，[具体药物名称]凝胶还使大鼠爪体积显著减小（p = 0.03）。由于慢性炎症，所有研究药物均使疼痛阈值（克/秒）显著降低（p < 0.05），但使用[具体药物名称]凝胶时，这种降低较少（p < 0.001）。
[具体药物名称]凝胶在慢性炎症大鼠模型局部应用时显示出显著的抗炎和轻度镇痛活性。
