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[在药物滥用范围内采用酶放大免疫分析技术(Emit ST)和薄层色谱法对尿液中的苯并二氮䓬类和噻吩并二氮䓬类药物进行筛查]

[Screening of benzo- and thienodiazepines in urine with Emit ST and thin-layer chromatography within the scope of substance abuse].

作者信息

Gruhl H

机构信息

Institut für Laboratoriumsmedizin, Zentralklinikum, Augsburg, Bundesrepublik Deutschland.

出版信息

Z Rechtsmed. 1987;98(4):221-8. doi: 10.1007/BF00201226.

Abstract

All 24 Benzo- and Thienodiazepines which are used in drugs in the FRG were added to drug free urine in different concentrations and examined by Emit ST as well as by TLC. In addition the acid hydrolysis products of diazepines were analysed by TLC. A modification of the extraction procedure is described, which shortens analysis time considerably. The detection limits were determined and it will be discussed how both methods, Emit and TLC, are suited for a wide-ranging drug screening program.

摘要

在联邦德国用于药物的所有24种苯并二氮䓬类和噻吩并二氮䓬类药物,被添加到不含药物的尿液中,浓度各异,并通过发射型计算机辅助扫描荧光计(Emit ST)以及薄层色谱法(TLC)进行检测。此外,通过薄层色谱法对二氮䓬类药物的酸水解产物进行了分析。文中描述了一种提取程序的改进方法,该方法可大幅缩短分析时间。确定了检测限,并将讨论发射型计算机辅助扫描荧光计和薄层色谱法这两种方法如何适用于广泛的药物筛查项目。

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