• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-203 抑制膀胱癌细胞生长并靶向 Twist1。

miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1.

机构信息

Department of Urology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, P.R. China.

Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, P.R. China.

出版信息

Oncol Res. 2018 Sep 14;26(8):1155-1165. doi: 10.3727/096504017X15041934685237. Epub 2017 Sep 6.

DOI:10.3727/096504017X15041934685237
PMID:28893347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7844766/
Abstract

miR-203 is an epigenetically silenced tumor-suppressive microRNA in tumors. This study was designed to investigate the effects of miR-203 on the proliferation, migration, invasion, and apoptosis of bladder cancer (BCa) cells. The expression levels of miR-203 in BCa tissues, normal adjacent tissues, and BCa cell lines were detected. BCa cells were transfected with miR-203 mimic and inhibitor to investigate the effect of miR-203 on cell functions and the epithelial-mesenchymal transition (EMT). Cotransfection with miR-203 inhibitor and shRNA of the predicted target gene Twist1 (si-Twist1) was performed to investigate the target relationship of miR-203 and Twist1. The effects of knockdown of Twist1 on cell functions were also investigated. The expression of miR-203 was significantly reduced in BCa tissues and cells, in comparison with the control. miR-203 mimic significantly reduced cell viability, invasion, migration, and EMT, and enhanced cell apoptosis. On the contrary, miR-203 inhibitor showed the opposite results. However, the administration of si-Twist1 cancelled the effect of miR-203 inhibitor on cell proliferation, apoptosis, invasion, and migration. These demonstrated that miR-203 may function as a tumor-suppressive microRNA in BCa by negatively targeting Twist1. Both Twist1 and miR-203 might be explored as potential targets for studying the mechanism related to BCa pathogenesis and therapy.

摘要

miR-203 是一种在肿瘤中被表观遗传沉默的肿瘤抑制性 microRNA。本研究旨在探讨 miR-203 对膀胱癌（BCa）细胞增殖、迁移、侵袭和凋亡的影响。检测了 miR-203 在 BCa 组织、正常相邻组织和 BCa 细胞系中的表达水平。转染 miR-203 模拟物和抑制剂以研究 miR-203 对细胞功能和上皮-间充质转化（EMT）的影响。转染 miR-203 抑制剂和预测靶基因 Twist1 的 shRNA（si-Twist1）以研究 miR-203 和 Twist1 的靶关系。还研究了敲低 Twist1 对细胞功能的影响。与对照组相比，miR-203 在 BCa 组织和细胞中的表达明显降低。miR-203 模拟物显著降低细胞活力、侵袭、迁移和 EMT，并增强细胞凋亡。相反，miR-203 抑制剂则表现出相反的结果。然而，si-Twist1 的给药取消了 miR-203 抑制剂对细胞增殖、凋亡、侵袭和迁移的影响。这些表明，miR-203 可能通过负向靶向 Twist1 发挥 BCa 中的肿瘤抑制性 microRNA 作用。Twist1 和 miR-203 都可能作为研究与 BCa 发病机制和治疗相关的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/c14e8d2d8dda/OR-26-1155-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/90d9539e9515/OR-26-1155-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/0fd899e6b9d6/OR-26-1155-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/f67e932c5c7b/OR-26-1155-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/be1fb3e0ede2/OR-26-1155-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/b4d4a423132e/OR-26-1155-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/c14e8d2d8dda/OR-26-1155-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/90d9539e9515/OR-26-1155-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/0fd899e6b9d6/OR-26-1155-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/f67e932c5c7b/OR-26-1155-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/be1fb3e0ede2/OR-26-1155-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/b4d4a423132e/OR-26-1155-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/231e/7844766/c14e8d2d8dda/OR-26-1155-g006.jpg

相似文献

1
miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1.miR-203 抑制膀胱癌细胞生长并靶向 Twist1。
Oncol Res. 2018 Sep 14;26(8):1155-1165. doi: 10.3727/096504017X15041934685237. Epub 2017 Sep 6.
2
microRNA-335 inhibits colorectal cancer HCT116 cells growth and epithelial-mesenchymal transition (EMT) process by targeting Twist1.微小RNA-335通过靶向Twist1抑制结直肠癌HCT116细胞的生长和上皮-间质转化（EMT）过程。
Pharmazie. 2017 Aug 1;72(8):475-481. doi: 10.1691/ph.2017.7489.
3
MicroRNA 495 Inhibits Proliferation and Metastasis and Promotes Apoptosis by Targeting Twist1 in Gastric Cancer Cells.MicroRNA 495 通过靶向 Twist1 抑制胃癌细胞的增殖、转移并促进凋亡。
Oncol Res. 2019 Feb 21;27(3):389-397. doi: 10.3727/096504018X15223159811838. Epub 2018 Apr 3.
4
Twist1-related miR-26b-5p suppresses epithelial-mesenchymal transition, migration and invasion by targeting SMAD1 in hepatocellular carcinoma.与Twist1相关的miR-26b-5p通过靶向SMAD1抑制肝细胞癌中的上皮-间质转化、迁移和侵袭。
Oncotarget. 2016 Apr 26;7(17):24383-401. doi: 10.18632/oncotarget.8328.
5
Circular RNA CNOT2 knockdown regulates twist family BHLH transcription factor via targeting microRNA 409-3p to prevent breast cancer invasion, migration and epithelial-mesenchymal transition.环状 RNA CNOT2 敲低通过靶向 microRNA 409-3p 调控 twist 家族 BHLH 转录因子，从而防止乳腺癌侵袭、迁移和上皮间质转化。
Bioengineered. 2021 Dec;12(1):9058-9069. doi: 10.1080/21655979.2021.1974805.
6
miR-326 regulates EMT and metastasis of endometrial cancer through targeting TWIST1.miR-326 通过靶向 TWIST1 调节子宫内膜癌的 EMT 和转移。
Eur Rev Med Pharmacol Sci. 2017 Oct;21(17):3787-3793.
7
MicroRNA-106b modulates epithelial-mesenchymal transition by targeting TWIST1 in invasive endometrial cancer cell lines.微小 RNA-106b 通过靶向 TWIST1 调节侵袭性子宫内膜癌细胞系中的上皮-间充质转化。
Mol Carcinog. 2014 May;53(5):349-59. doi: 10.1002/mc.21983. Epub 2013 Sep 3.
8
MiR-15a-3p suppresses the growth and metastasis of ovarian cancer cell by targeting Twist1.miR-15a-3p 通过靶向 Twist1 抑制卵巢癌细胞的生长和转移。
Eur Rev Med Pharmacol Sci. 2019 Mar;23(5):1934-1946. doi: 10.26355/eurrev_201903_17232.
9
TWIST1 upregulates miR-214 to promote epithelial-to-mesenchymal transition and metastasis in lung adenocarcinoma.TWIST1 通过上调 miR-214 促进肺腺癌的上皮间质转化和转移。
Int J Mol Med. 2018 Jul;42(1):461-470. doi: 10.3892/ijmm.2018.3630. Epub 2018 Apr 17.
10
CASC15 promotes epithelial to mesenchymal transition and facilitates malignancy of hepatocellular carcinoma cells by increasing TWIST1 gene expression via miR-33a-5p sponging.CASC15 通过海绵吸附 miR-33a-5p 增加 TWIST1 基因表达促进上皮间质转化并促进肝癌细胞的恶性转化。
Eur J Pharmacol. 2019 Oct 5;860:172589. doi: 10.1016/j.ejphar.2019.172589. Epub 2019 Aug 8.

引用本文的文献

1
Extracellular vesicles-derived extracellular RNA in bladder cancer: current progress and future prospects.膀胱癌中细胞外囊泡衍生的细胞外RNA：当前进展与未来展望
Ther Adv Med Oncol. 2025 Jul 23;17:17588359251349288. doi: 10.1177/17588359251349288. eCollection 2025.
2
circICMT upregulates and suppresses the malignant behavior of bladder cancer.环状异戊烯基半胱氨酸羧甲基转移酶上调并抑制膀胱癌的恶性行为。
Transl Oncol. 2025 Feb;52:102262. doi: 10.1016/j.tranon.2024.102262. Epub 2024 Dec 28.
3
Bladder cancer: non-coding RNAs and exosomal non-coding RNAs.

本文引用的文献

1
MicroRNA-383 is a tumor suppressor and potential prognostic biomarker in human non-small cell lung caner.微小 RNA-383 是人类非小细胞肺癌的肿瘤抑制因子和潜在的预后生物标志物。
Biomed Pharmacother. 2016 Oct;83:1175-1181. doi: 10.1016/j.biopha.2016.08.006. Epub 2016 Aug 20.
2
Specific miRNA Disease Biomarkers in Blood, Serum and Plasma: Challenges and Prospects.血液、血清和血浆中的特定miRNA疾病生物标志物：挑战与前景
Mol Diagn Ther. 2016 Dec;20(6):509-518. doi: 10.1007/s40291-016-0221-4.
3
Loss of miR-203 regulates cell shape and matrix adhesion through ROBO1/Rac/FAK in response to stiffness.
膀胱癌：非编码 RNA 和外泌体非编码 RNA。
Funct Integr Genomics. 2024 Aug 31;24(5):147. doi: 10.1007/s10142-024-01433-9.
4
CREB1 activation promotes human papillomavirus oncogene expression and cervical cancer cell transformation.CREB1 激活促进人乳头瘤病毒癌基因表达和宫颈癌细胞转化。
J Med Virol. 2023 Aug;95(8):e29025. doi: 10.1002/jmv.29025.
5
microRNA-203 functions as a natural Ras inhibitor in hepatocellular carcinoma.微小RNA-203在肝细胞癌中作为一种天然的Ras抑制剂发挥作用。
Am J Cancer Res. 2023 Apr 15;13(4):1295-1309. eCollection 2023.
6
Circular RNAs: pivotal role in the leukemogenesis and novel indicators for the diagnosis and prognosis of acute myeloid leukemia.环状RNA：在白血病发生中的关键作用及急性髓系白血病诊断和预后的新指标
Front Oncol. 2023 Apr 14;13:1149187. doi: 10.3389/fonc.2023.1149187. eCollection 2023.
7
The Role of MicroRNA in the Regulation of Tumor Epithelial-Mesenchymal Transition.miRNA 在肿瘤上皮间质转化调控中的作用。
Cells. 2022 Jun 21;11(13):1981. doi: 10.3390/cells11131981.
8
Exosome-mediated miR-25/miR-203 as a potential biomarker for esophageal squamous cell carcinoma: improving early diagnosis and revealing malignancy.外泌体介导的miR-25/miR-203作为食管鳞状细胞癌的潜在生物标志物：改善早期诊断并揭示恶性程度
Transl Cancer Res. 2021 Dec;10(12):5174-5182. doi: 10.21037/tcr-21-1123.
9
Long non-coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA-299-3p expression.长链非编码RNA UCA1通过调控微小RNA-299-3p的表达促进宫颈癌细胞的增殖和侵袭。
Oncol Lett. 2021 Nov;22(5):772. doi: 10.3892/ol.2021.13033. Epub 2021 Sep 9.
10
HOTAIR Contributes to Stemness Acquisition of Cervical Cancer through Regulating miR-203 Interaction with ZEB1 on Epithelial-Mesenchymal Transition.HOTAIR通过调控miR-203与ZEB1在上皮-间质转化中的相互作用促进宫颈癌干性的获得。
J Oncol. 2021 Sep 10;2021:4190764. doi: 10.1155/2021/4190764. eCollection 2021.
miR-203的缺失通过ROBO1/Rac/FAK响应硬度调节细胞形状和基质粘附。
J Cell Biol. 2016 Mar 14;212(6):707-19. doi: 10.1083/jcb.201507054.
4
Role of long non-coding RNA MIAT in proliferation, apoptosis and migration of lens epithelial cells: a clinical and in vitro study.长链非编码RNA MIAT在晶状体上皮细胞增殖、凋亡和迁移中的作用：一项临床和体外研究
J Cell Mol Med. 2016 Mar;20(3):537-48. doi: 10.1111/jcmm.12755. Epub 2016 Jan 28.
5
Hypoxia promotes vasculogenic mimicry formation by the Twist1-Bmi1 connection in hepatocellular carcinoma.缺氧通过Twist1-Bmi1连接促进肝细胞癌中血管生成拟态的形成。
Int J Mol Med. 2015 Sep;36(3):783-91. doi: 10.3892/ijmm.2015.2293. Epub 2015 Jul 22.
6
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.细胞质中的p27通过STAT3介导的Twist1上调促进上皮-间质转化和肿瘤转移。
Oncogene. 2015 Oct;34(43):5447-59. doi: 10.1038/onc.2014.473. Epub 2015 Feb 16.
7
Integrating the roles of long and small non-coding RNA in brain function and disease.长链和短非编码 RNA 在脑功能和疾病中的作用整合。
Mol Psychiatry. 2014 Apr;19(4):410-6. doi: 10.1038/mp.2013.196. Epub 2014 Jan 28.
8
miR-203 inhibits the proliferation and self-renewal of esophageal cancer stem-like cells by suppressing stem renewal factor Bmi-1.微小RNA-203通过抑制干细胞更新因子Bmi-1来抑制食管癌干细胞样细胞的增殖和自我更新。
Stem Cells Dev. 2014 Mar 15;23(6):576-85. doi: 10.1089/scd.2013.0308. Epub 2014 Jan 4.
9
miR-203 induces oxaliplatin resistance in colorectal cancer cells by negatively regulating ATM kinase.微小RNA-203通过负向调控ATM激酶诱导大肠癌细胞对奥沙利铂产生耐药性。
Mol Oncol. 2014 Feb;8(1):83-92. doi: 10.1016/j.molonc.2013.09.004. Epub 2013 Oct 8.
10
MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met.MicroRNA-409-3p 通过靶向 c-Met 抑制膀胱癌细胞的迁移和侵袭。
Mol Cells. 2013 Jul;36(1):62-8. doi: 10.1007/s10059-013-0044-7. Epub 2013 May 30.