Sun Cong, Wu Chen, Su Yue, Wang Rui-Jun, Fu Ge-Yi, Zhao Zhe, Yu Xiao-Yun, Huang Meng-Meng, Han Bing-Nan, Lv Zheng-Bing, Wu Min
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, PR China.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4024-4031. doi: 10.1099/ijsem.0.002237. Epub 2017 Sep 12.
A Gram-staining-negative, aerobic, non-spore-forming, coccoid to rod shaped bacteria with prosthecate and flagellum, designated as HSF6, was isolated from deep seawater samples collected from the South China Sea at depth of 2.5 km and subjected to a polyphasic taxonomic investigation. Colonies of strain HSF6 were 1-2 mm in diameter, smooth, circular, convex and yellow. Strain HSF6 was found to grow at 15-37 °C (optimum, 25-35 °C), pH 5.0-9.5 (optimum, pH 7.0-7.5) and with 0-8 % (w/v) NaCl (optimum, 2 %). Chemotaxonomic analysis showed the predominant respiratory quinone of strains HSF6 were ubiquinone-10, and the major fatty acids were C18 : 1ω7c, C16 : 0 and 11-methyl C18 : 1ω7c. The polar lipids were monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), three unknown glycolipids (GL1-3) and five unknown lipids (L1-5). The DNA G+C content of strain HSF6 was determined to be 51.0 mol% with HPLC. The comparison of 16S rRNA gene sequence similarities show that strain HSF6 was related most closely to genus Parvularcula with similarity ranging from 91.0 to 91.8 %. The phylogenetic trees, using the 16S rRNA gene sequence, reconstructed with neighbour-joining, maximum-parsimony and maximum-likelihood methods showed that strain HSF6 constituted a separated branch in the family 'Parvularculaceae'. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain HSF6 is clearly distinct from validly published genera. On the basis of these features, we propose strain HSF6 (=MCCC 1K03223=KCTC 52486) represents a novel species of a novel genus with the name Hyphococcus flavus gen. nov., sp. nov.
从南海2.5千米深处采集的深海海水样本中分离出一株革兰氏染色阴性、需氧、无芽孢、具菌柄和鞭毛、呈球状至杆状的细菌，命名为HSF6，并对其进行了多相分类学研究。HSF6菌株的菌落直径为1-2毫米，光滑、圆形、凸起且呈黄色。发现HSF6菌株可在15-37°C（最适温度为25-35°C）、pH值5.0-9.5（最适pH值为7.0-7.5）以及0-8%（w/v）NaCl（最适浓度为2%）的条件下生长。化学分类分析表明，HSF6菌株的主要呼吸醌为泛醌-10，主要脂肪酸为C18:1ω7c、C16:0和11-甲基C18:1ω7c。极性脂类为单半乳糖二酰甘油（MGDG）、磺基喹喔啉二酰甘油（SQDG）、三种未知糖脂（GL1-3）和五种未知脂类（L1-5）。利用高效液相色谱法测定HSF6菌株的DNA G+C含量为51.0摩尔%。16S rRNA基因序列相似性比较表明，HSF6菌株与微小杆菌属的亲缘关系最为密切，相似性范围为91.0%至91.8%。使用邻接法、最大简约法和最大似然法重建的基于16S rRNA基因序列的系统发育树表明，HSF6菌株在“微小杆菌科”中构成一个独立分支。不同的表型特征以及系统发育独特性表明，HSF6菌株明显不同于已有效发表的属。基于这些特征，我们提议HSF6菌株（=MCCC 1K03223=KCTC 52486）代表一个新属的新物种，命名为黄色丝球菌属（Hyphococcus flavus），新属，新种。
