Department of Marine Pharmacy, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, People's Republic of China.
Ningbo Institute of Marine Medicine, Peking University, Ningbo, 315800, Zhejiang, People's Republic of China.
Antonie Van Leeuwenhoek. 2023 Jul;116(7):711-719. doi: 10.1007/s10482-023-01838-9. Epub 2023 May 10.
A novel strain designated NBU1457, was isolated from marine sediment sampled on Meishan Island located in the East China Sea. Cells of strain NBU1457 was Gram-negative, facultatively anaerobic, non-motile and ovoid-shaped. Strain NBU1457 grew optimally at 37 °C, NaCl concentration of 2.0-3.0% (w/v) and pH 6.5-7.5. Catalase and oxidase activities, urease, nitrate reduction and HS production were positive. Indole production, methyl red reaction, hydrolysis of starch, gelatin, casein, Tweens 20, 40, 60 and 80 were negative. Comparative analysis of the 16S rRNA gene sequence showed highest similarities to the species with validated name Oricola thermophila MEBiC13590 (98.8%), Oricola cellulosilytica CC-AMH-0 (97.9%) and Oricola indica JL-62 (97.9%). Phylogenetic analyses indicated that strain NBU1457 clustered with the genus Oricola and closely related to strains Oricola thermophila MEBiC13590, Oricola cellulosilytica CC-AMH-0 and Oricola indica JL-62. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1457 and related species of genus Oricola were well below the threshold limit for prokaryotic species delineation. The DNA G + C content was 63.2%. The major cellular fatty acid was summed feature 8 (C ω7c and/or C ω6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, sulfoquinovosyldiacylglycerol and phosphatidylglycerol. The only respiratory quinone was ubiquinone-10 (Q-10). Combining results of our phenotypic, chemotaxonomic and genotypic data, strain NBU1457 is considered to be a representative in the genus Oricola, which the name Oricola nitratireducens sp. nov. is proposed. The type strain of the new species is NBU1457 (= KCTC 82225 = MCCC 1K04764).
一株新的菌株，命名为 NBU1457，从位于东海的梅山岛的海洋沉积物中分离出来。菌株 NBU1457 的细胞为革兰氏阴性、兼性厌氧、非运动性和卵圆形。菌株 NBU1457 在 37°C、2.0-3.0%（w/v）NaCl 浓度和 pH 6.5-7.5 条件下生长最佳。该菌株具有过氧化氢酶和氧化酶活性、脲酶、硝酸盐还原和 H2S 生成阳性，吲哚生成、甲基红反应、淀粉、明胶、酪蛋白、吐温 20、40、60 和 80 水解阴性。16S rRNA 基因序列的比较分析显示，与经证实的种 Oricola thermophila MEBiC13590（98.8%）、Oricola cellulosilytica CC-AMH-0（97.9%）和 Oricola indica JL-62（97.9%）的相似度最高。系统发育分析表明，菌株 NBU1457 与 Oricola 属聚类，并与菌株 Oricola thermophila MEBiC13590、Oricola cellulosilytica CC-AMH-0 和 Oricola indica JL-62 密切相关。菌株 NBU1457 与 Oricola 属相关种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值远低于原核种划分的阈值。DNA G+C 含量为 63.2%。主要细胞脂肪酸为 summed feature 8（C ω7c 和/或 C ω6c）。主要极性脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂单甲基乙醇胺、磺基奎诺糖二酰基甘油和磷脂酰甘油。唯一的呼吸醌为泛醌-10（Q-10）。结合表型、化学分类和基因型数据的结果，菌株 NBU1457 被认为是 Oricola 属的一个代表种，因此建议命名为 Oricola nitratireducens sp. nov.。新种的模式菌株为 NBU1457（=KCTC 82225=MCCC 1K04764）。
