Martínez Rebeca, Hernández Liz, Gil Lázaro, Carpio Yamila, Morales Antonio, Herrera Fidel, Rodríguez-Mallón Alina, Leal Yeny, Blanco Aracelys, Estrada Mario Pablo
Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Vaccine Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Vaccine. 2017 Oct 9;35(42):5722-5728. doi: 10.1016/j.vaccine.2017.07.060.
Modern subunit vaccines have excellent safety profiles and improved tolerability, but do not elicit strong immune responses without the addition of adjuvants. Developing a safe and affective adjuvant remains a challenge for peptide-based vaccine design. Growth Hormone Releasing Peptide-6 (GHRP-6) is one of the earliest-developed, synthetic, peptidyl growth hormone secretagogue receptor agonists. These compounds mimic the effect of the endogenous ligand, ghrelin. In the present study, we evaluated the ability of GHRP-6 to enhance the humoral immune response against co-injected antigens in mice, tilapia and African catfish. This peptide was able to increase the antigen-specific antibody response using heterologous proteins and peptides as antigens, which were also formulated in "water in oil" emulsions (Freund and Montanide). As long as we know there is no previous report describing any ghrelin analogous as molecular immunomodulator stimulating a humoral immune response. Further studies will be conducted to evaluate the functionality of this humoral immune response in challenge trials.
现代亚单位疫苗具有出色的安全性和更好的耐受性，但在不添加佐剂的情况下不会引发强烈的免疫反应。开发一种安全有效的佐剂仍然是基于肽的疫苗设计面临的一项挑战。生长激素释放肽-6（GHRP-6）是最早开发的合成肽基生长激素促分泌素受体激动剂之一。这些化合物模拟内源性配体胃泌素的作用。在本研究中，我们评估了GHRP-6增强小鼠、罗非鱼和非洲鲶鱼针对共注射抗原的体液免疫反应的能力。该肽能够使用异源蛋白质和肽作为抗原增加抗原特异性抗体反应，这些抗原也被制成“油包水”乳剂（弗氏佐剂和蒙塔纳ide佐剂）。据我们所知，此前没有任何关于胃泌素类似物作为刺激体液免疫反应的分子免疫调节剂的报道。将进行进一步研究以评估这种体液免疫反应在攻毒试验中的功能。
