Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba.
Vaccine Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba.
Vaccine. 2014 Jan 3;32(2):223-9. doi: 10.1016/j.vaccine.2013.11.009. Epub 2013 Nov 17.
Modern vaccines based on purified recombinant antigens have improved their safety; however they induce a suboptimal immune response without the help of adjuvants. Consequently, the development of new adjuvants to enhance the immunogenicity of purified subunit antigens and modulate resulting immune responses is of great interest. In the present study, we evaluated the ability of antimicrobial peptides Oreochromicins previously isolated from tilapia Oreochromis niloticus to enhance adaptive immune responses in mice and tilapia. When co-administrated with ovalbumin in mice, Oreochromicin-1 induced a TH1 humoral immune response. Oreochromicin-2 and 3 induce a TH1 cellular immune response characterized by the induction of interferon-γ in a dose depend manner. Additionally, co-administration of Oreochromicin-1 with the sea lice my32 from Lepeophtheirus salmonis antigen (my32-Ls) increases the humoral immune response in mice and tilapia. We also tested different combinations of these Oreochromicins with the sea lice antigen my32-Ls in mice. Humoral and cellular TH1 responses were enhanced by co-administration of my32-Ls/Oreochromicin-3 and the combination my32-Ls/Oreochromicin-2/3. In agreement with these results, Oreochromicin-1 and 3 enhanced in vitro TH1 cytokine IFN-γ production in Concanavalin A primed splenocytes from naïve mice after a 48h incubation period. In summary, the results showed that tilapia alpha-helical antimicrobial peptides Oreochromicins are able to boost immune response in mammals and fish, encouraging their use as TH1 molecular adjuvants to subunit antigens.
基于纯化重组抗原的现代疫苗提高了安全性；然而，在没有佐剂的帮助下，它们会引起不理想的免疫反应。因此，开发新的佐剂来增强纯化亚单位抗原的免疫原性并调节由此产生的免疫反应具有重要意义。在本研究中，我们评估了先前从罗非鱼 Oreochromis niloticus 中分离出的抗菌肽 Oreochromicins 增强小鼠和罗非鱼适应性免疫反应的能力。当与卵清蛋白在小鼠中共同给药时，Oreochromicin-1 诱导 TH1 体液免疫反应。Oreochromicin-2 和 3 诱导 TH1 细胞免疫反应，其特征在于干扰素-γ的诱导呈剂量依赖性。此外，Oreochromicin-1 与鲑鱼海虱 My32 抗原（my32-Ls）共同给药可增加小鼠和罗非鱼的体液免疫反应。我们还在小鼠中测试了这些 Oreochromicins 与海虱抗原 my32-Ls 的不同组合。共给药 my32-Ls/Oreochromicin-3 和组合 my32-Ls/Oreochromicin-2/3 增强了体液和细胞 TH1 反应。与这些结果一致，Oreochromicin-1 和 3 增强了在 Concanavalin A 诱导的来自幼稚小鼠的脾细胞中 48 小时孵育后 TH1 细胞因子 IFN-γ的体外产生。总之，结果表明，罗非鱼α-螺旋抗菌肽 Oreochromicins 能够增强哺乳动物和鱼类的免疫反应，鼓励将其用作亚单位抗原的 TH1 分子佐剂。
