Watanabe Satoko, Iizuka Tetsuya, Hatama Shinichi, Kanno Toru, Mase Masaji, Shibahara Tomoyuki
National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Institute of Agrobiological Science, NARO, 2-1 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
Vaccine. 2017 Oct 13;35(43):5878-5882. doi: 10.1016/j.vaccine.2017.08.079. Epub 2017 Sep 8.
Bovine papillomaviruses (BPVs) are the causative agent of bovine teat papillomatosis, which can lead to severe economic losses in dairy cattle. Among the 14 identified BPV genotypes, BPV type 6 (BPV6) is the most frequently detected in teat papilloma lesions, and is therefore thought to play a major role in teat papillomatosis. To develop an effective vaccine against BPV6 infection, we produced virus-like particles of BPV6 (BPV6-VLP) in silkworm (Bombyx mori) pupae and purified these by heparin affinity chromatography using a single column. About 0.7mg purified BPV6-VLP was obtained from one pupa. BPV6-VLP-immunized mice produced a specific IgG to BPV6 that recognized BPV6 antigen with high sensitivity in an immunohistochemical analysis. Thus, silkworm pupae are a useful bioreactor for the production of BPV6-VLP, which can potentially be used as a vaccine for bovine teat papillomatosis.
牛乳头瘤病毒（BPVs）是牛乳头瘤病的病原体，可给奶牛造成严重的经济损失。在已鉴定出的14种BPV基因型中，BPV 6型（BPV6）是在乳头瘤病变中最常检测到的，因此被认为在牛乳头瘤病中起主要作用。为开发一种针对BPV6感染的有效疫苗，我们在蚕（家蚕）蛹中生产了BPV6病毒样颗粒（BPV6-VLP），并使用单一柱通过肝素亲和色谱法对其进行了纯化。从一个蛹中可获得约0.7mg纯化的BPV6-VLP。用BPV6-VLP免疫的小鼠产生了针对BPV6的特异性IgG，该IgG在免疫组织化学分析中能以高灵敏度识别BPV6抗原。因此，蚕蛹是生产BPV6-VLP的有用生物反应器，BPV6-VLP有可能用作牛乳头瘤病的疫苗。
