Department of Virology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Virology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Microb Pathog. 2022 Sep;170:105713. doi: 10.1016/j.micpath.2022.105713. Epub 2022 Aug 14.
Papillomaviruses are epitheliotropic in nature and cause proliferation in the skin, mucosa, and various internal organs of various animal species. The lesions they cause, specifically in cattle teats, lead to significant economic losses in the milk industry. In this study, we identified the bovine papillomaviruses (BPVs) responsible for teat papillomas in cattle. The tissue damage caused by the virus was examined histopathologically using immunohistochemical, transmission electron microscopy (TEM), and molecular methods. Additionally, sequence analyses were performed on the isolated field strains to better understand their genetic and phylogenetic relationships with previously reported isolates. Teat papillomatosis was confirmed in the collected samples by histopathological and immunohistochemical methods, which were followed by other diagnostic methods. Intranuclear virus particles were found in the epithelial cells during a TEM examination of teat lesions. BPV was detected in seven samples by performing PCR using degenerate primers and specific primers. The positive samples were used for typing through sequence analysis/PCR with type-specific primers. Three isolates from teat tissues with BPV infection were identified as BPV-6, two as BPV-10, one as BPV-2, and one as BPV-8. The five isolates identified through sequence analysis of positive samples belonged to the Xipapillomavirus 1 genus (one), the Epsilonpapillomavirus 1 genus (one), and the Deltapapillomavirus genus (one) (three). Furthermore, type-specific primers were found to be useful for molecular diagnosis of BPV, which occurs in the etiology of teat papillomas, followed by genotyping and primer generation during characterization. The detection of BPV types and their prevalence, biosafety measures in animal breeding, and the importance of vaccine research are all important.
乳头瘤病毒本质上是上皮嗜性的，会导致各种动物物种的皮肤、黏膜和各种内部器官增生。它们引起的病变，特别是在奶牛的乳头上，会给牛奶产业造成巨大的经济损失。在本研究中，我们鉴定了导致奶牛乳头瘤的牛乳头瘤病毒（BPV）。使用免疫组织化学、透射电子显微镜（TEM）和分子方法对病毒引起的组织损伤进行了组织病理学检查。此外，还对分离的田间株进行了序列分析，以更好地了解它们与以前报道的分离株的遗传和系统发育关系。通过组织病理学和免疫组织化学方法在收集的样本中确认了乳头瘤病，随后进行了其他诊断方法。在对乳头病变进行 TEM 检查时发现上皮细胞中有核内病毒颗粒。通过使用简并引物和特异性引物进行 PCR，在七个样本中检测到 BPV。用阳性样本进行分型，通过与型特异性引物进行序列分析/PCR。从感染 BPV 的乳头组织中分离出三个分离株，鉴定为 BPV-6、两个为 BPV-10、一个为 BPV-2 和一个为 BPV-8。通过对阳性样本进行序列分析鉴定出的五个分离株属于 Xipapillomavirus 1 属（一个）、Epsilonpapillomavirus 1 属（一个）和 Deltapapillomavirus 属（三个）。此外，发现型特异性引物对分子诊断 BPV 非常有用，BPV 是引起乳头瘤的病因之一，随后在特征描述时进行了基因分型和引物生成。BPV 类型的检测及其流行率、动物养殖中的生物安全措施以及疫苗研究的重要性都非常重要。
