Ichijo Ryo, Kobayashi Hiroki, Yoneda Saori, Iizuka Yui, Kubo Hirokazu, Matsumura Shigeru, Kitano Satsuki, Miyachi Hitoshi, Honda Tetsuya, Toyoshima Fumiko
Department of Biosystems Science, Institute for Frontier Life and Medical Science, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.
Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Nat Commun. 2017 Sep 11;8(1):508. doi: 10.1038/s41467-017-00433-7.
The skin surface area varies flexibly in response to body shape changes. Skin homeostasis is maintained by stem cells residing in the basal layer of the interfollicular epidermis. However, how the interfollicular epidermal stem cells response to physiological body shape changes remains elusive. Here, we identify a highly proliferative interfollicular epidermal basal cell population in the rapidly expanding abdominal skin of pregnant mice. These cells express Tbx3 that is necessary for their propagation to drive skin expansion. The Tbx3 basal cells are generated from Axin2 interfollicular epidermal stem cells through planar-oriented asymmetric or symmetric cell divisions, and express transit-amplifying cell marker CD71. This biased division of Axin2 interfollicular epidermal stem cells is induced by Sfrp1 and Igfbp2 proteins secreted from dermal cells. The Tbx3 basal cells promote wound repair, which is enhanced by Sfrp1 and Igfbp2. This study elucidates the interfollicular epidermal stem cell/progeny organisation during pregnancy and suggests its application in regenerative medicine.The abdominal skin expands rapidly during pregnancy. Here the authors show that a population of highly proliferative stem cell progenies expressing the transcription factor Tbx3 is required for abdominal skin expansion in pregnant mice.
皮肤表面积会根据身体形状的变化而灵活改变。皮肤稳态由位于毛囊间表皮基底层的干细胞维持。然而，毛囊间表皮干细胞如何响应生理性身体形状变化仍不清楚。在此，我们在怀孕小鼠快速扩张的腹部皮肤中鉴定出一群高度增殖的毛囊间表皮基底细胞。这些细胞表达Tbx3，这是它们增殖以驱动皮肤扩张所必需的。Tbx3基底细胞由Axin2毛囊间表皮干细胞通过平面定向的不对称或对称细胞分裂产生，并表达过渡放大细胞标志物CD71。Axin2毛囊间表皮干细胞的这种偏向性分裂由真皮细胞分泌的Sfrp1和Igfbp2蛋白诱导。Tbx3基底细胞促进伤口修复，Sfrp1和Igfbp2可增强这种修复。这项研究阐明了怀孕期间毛囊间表皮干细胞/子代的组织情况，并提出了其在再生医学中的应用。怀孕期间腹部皮肤迅速扩张。在此，作者表明，一群表达转录因子Tbx3的高度增殖干细胞子代是怀孕小鼠腹部皮肤扩张所必需的。
