Abe K, Takano H
Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan.
Arch Histol Jpn. 1987 Jul;50(3):315-24. doi: 10.1679/aohc.50.315.
Cryptorchid surgery to move the testis and epididymis to the abdomen was performed in mice of 60 days of age; one week later the mice were subjected to further surgery to reposition the organs in the scrotum. The mice were sacrificed at 1 week intervals until 11 weeks after the initial operation. After the first operation, spermatozoa in the epididymal duct rapidly disappeared and were almost absent until the 4th week. Then spermatozoa appeared again, increased, and reached normal numbers by 10 weeks. With the disappearance of the spermatozoa, a PAS-positive material, which is believed to be secreted in the middle part of the head of the epididymal duct (ABE et al., 1982), was accumulated in the epididymal duct in the body and tail of the epididymis, and PAS-positive inclusions appeared in the principal cells of the duct in the body 2 weeks after the first operation. The inclusions developed in size and number during the aspermia period, then decreased in number with the reappearance of spermatozoa from the 5th week to ultimately disappear by 10 weeks after the first operation. We have previously demonstrated that such inclusions appear also after efferent duct ligation, which interrupts the flow of spermatozoa and testicular fluid into the epididymal duct (ABE et al., 1982). Both present and previous findings indicate that the appearance of PAS-positive inclusions depends on the absence of spermatozoa in the epididymal duct irrespective of the presence of the testicular fluid in the duct. It is suggested that the PAS-positive material is utilized by spermatozoa and, in the absence of spermatozoa, is accumulated in the lumen and ingested by the principal cells of the epididymal duct in the body of the epididymis.
对60日龄小鼠进行隐睾手术，将睾丸和附睾移至腹腔；一周后，对小鼠进行进一步手术，将这些器官重新置于阴囊中。每隔1周处死小鼠，直至初次手术后11周。首次手术后，附睾管内的精子迅速消失，直到第4周几乎都不存在。然后精子再次出现并增多，到10周时达到正常数量。随着精子消失，一种PAS阳性物质（据信是在附睾管头部中部分泌的，ABE等人，1982年）在附睾体部和尾部的附睾管内积聚，并且在首次手术后2周，附睾体部管道的主细胞中出现PAS阳性包涵体。在无精子期，这些包涵体的大小和数量不断增加，然后随着精子从第5周再次出现，其数量减少，到首次手术后10周最终消失。我们之前已经证明，在输出小管结扎后也会出现这种包涵体，输出小管结扎会中断精子和睾丸液流入附睾管（ABE等人，1982年）。目前和之前的研究结果均表明，PAS阳性包涵体的出现取决于附睾管内精子的缺失，而与管内睾丸液的存在无关。有人认为，PAS阳性物质被精子利用，在没有精子的情况下，会在管腔内积聚并被附睾体部附睾管的主细胞摄取。
