Abe K, Takano H
Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan.
Arch Histol Cytol. 1988 Dec;51(5):433-41. doi: 10.1679/aohc.51.433.
The distribution of spermatozoa and that as well as the staining reaction of PAS-positive material in the epididymal duct were histologically observed in 60 day-old mice and mice 12 h, 24 h, 2 days, and 7 days after efferent duct ligation at 60 days of age. We divided the mouse epididymal duct into five segments; Segments I, II, and III making up the head of the epididymis, Segment IV the body, and Segment V the tail. The cytoplasm of the principal cells in Segment II was PAS-positive. The lumen of the epididymal duct contained spermatozoa and PAS-positive material in the distal portion of Segment I and other segments distal to Segment I. The lumen in Segments IV and V was distended with abundant spermatozoa and PAS-positive material. After efferent duct ligation, spermatozoa disappeared from the lumen in Segments I, II, and III within 2 days, whereas they took 5 days to pass from Segment IV. As spermatozoa disappeared, the luminal material increased in intensity of the PAS-reaction in the distal portion of Segment I and initial portion of Segment II and Segment IV. Segment I became similar in appearance to Segment II 7 days after the ligation. The findings suggest that the PAS-positive material is produced in the distal portion of Segment I and Segment II and accumulates, in the absence of spermatozoa, at the production site and Segment IV, a storage site of spermatozoa; furthermore, Segment I has the capacity of exerting a Segment II-like function, which becomes distinct after blocking the entrance of the testicular fluid.
对60日龄小鼠以及60日龄时进行输出小管结扎后12小时、24小时、2天和7天的小鼠，进行了附睾管内精子的分布以及PAS阳性物质染色反应的组织学观察。我们将小鼠附睾管分为五个节段；节段I、II和III构成附睾头部，节段IV为体部，节段V为尾部。节段II主细胞的细胞质呈PAS阳性。附睾管腔在节段I的远端部分以及节段I远端的其他节段含有精子和PAS阳性物质。节段IV和V的管腔内充满了大量精子和PAS阳性物质。输出小管结扎后，节段I、II和III管腔内的精子在2天内消失，而节段IV的精子则需5天才能排空。随着精子消失，节段I远端部分、节段II起始部分和节段IV管腔内物质的PAS反应强度增加。结扎7天后，节段I的外观与节段II相似。这些发现表明，PAS阳性物质在节段I和节段II的远端部分产生，在没有精子的情况下，在产生部位和精子储存部位节段IV积累；此外，节段I具有发挥类似节段II功能的能力，在阻断睾丸液进入后这种功能变得明显。
