Abe K, Takano H, Ito T
Anat Rec. 1983 Sep;207(1):17-24. doi: 10.1002/ar.1092070103.
The mouse epididymal duct can be histologically divided into five segments (I-V), and the principal cells in segment II appear to secrete periodic acid-Schiff (PAS)-positive material into the lumen. In this study, male dd-mice received one, two, or four 800-R doses of radiation beginning at age 50 days. Mice receiving multiple doses were irradiated at 1-week intervals. After irradiation, marked depletion of spermatozoa, or aspermia, occurred in the epididymal duct for 2 to 16 weeks after a latency period of 3 to 4 weeks according to the times of irradiations. During oligospermia or aspermia, PAS-positive inclusions appeared in the principal cells in segment IV. The inclusions occupied a supranuclear position and appeared as round granules and globules measuring 2-15 micron in diameter, and increased in number, size, and staining intensity with time. They disappeared after reappearance of spermatozoa. The findings suggest that PAS-positive material may bind to spermatozoa and, if not bound, is reabsorbed by the principal cells in segment IV and deposited as intracellular inclusions, and the principal cells in segment IV are capable of digesting the accumulated PAS-positive material.
小鼠附睾管在组织学上可分为五个节段（I-V），节段II中的主细胞似乎向管腔分泌过碘酸希夫（PAS）阳性物质。在本研究中，雄性dd小鼠从50日龄开始接受1次、2次或4次800伦琴剂量的辐射。接受多次辐射的小鼠每隔1周进行一次照射。照射后，根据照射次数，在3至4周的潜伏期后，附睾管中出现精子明显减少或无精子症，持续2至16周。在少精子症或无精子症期间，节段IV的主细胞中出现PAS阳性包涵体。这些包涵体位于核上位置，呈圆形颗粒和小球状，直径为2-15微米，并随着时间的推移数量、大小和染色强度增加。精子再次出现后它们消失。这些发现表明，PAS阳性物质可能与精子结合，如果未结合，则被节段IV的主细胞重新吸收并沉积为细胞内包涵体，并且节段IV的主细胞能够消化积累的PAS阳性物质。
