Suh Kwang Sik, Chon Suk, Choi Eun Mi
Department of Endocrinology and Metabolism, School of Medicine, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
Cytotechnology. 2018 Feb;70(1):215-224. doi: 10.1007/s10616-017-0135-y. Epub 2017 Sep 11.
Bergenin, an active component of plants in the genus Bergenia, has multiple biological activities, including anti-inflammatory and immunomodulatory properties. We investigated the effects of bergenin on MC3T3-E1 osteoblasts. Bergenin treatment significantly elevated collagen synthesis, alkaline phosphatase activity, osteocalcin synthesis, and mineralization in the cells (p < 0.05). Additionally, bergenin increased the ratio of osteoprotegerin to receptor activator of nuclear factor kappa-B ligand, and cyclophilin B release. Methylglyoxal (MG), a highly reactive dicarbonyl compound, is the major precursor in the formation of advanced glycation end products. Pretreatment of MC3T3-E1 cells with bergenin prevented MG-induced cell death. Furthermore, bergenin treatment significantly reduced the induction of activating transcription factor 6 and autophagy by MG. These results indicate that bergenin may have positive effects on critical osteoblastic cell functions.
岩白菜素是虎耳草属植物中的一种活性成分，具有多种生物活性，包括抗炎和免疫调节特性。我们研究了岩白菜素对MC3T3-E1成骨细胞的影响。岩白菜素处理显著提高了细胞中的胶原蛋白合成、碱性磷酸酶活性、骨钙素合成和矿化作用（p < 0.05）。此外，岩白菜素增加了骨保护素与核因子κB受体激活剂配体的比例以及亲环素B的释放。甲基乙二醛（MG）是一种高反应性二羰基化合物，是晚期糖基化终末产物形成的主要前体。用岩白菜素预处理MC3T3-E1细胞可防止MG诱导的细胞死亡。此外，岩白菜素处理显著降低了MG对激活转录因子6的诱导作用和自噬作用。这些结果表明，岩白菜素可能对关键的成骨细胞功能具有积极作用。
