Hou Weiduo, Ye Chenyi, Chen Mo, Li Weixu, Gao Xiang, He Rongxin, Zheng Qiang, Zhang Wei
Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Research institute of Orthopaedics, Zhejiang University, Hangzhou, China.
Front Pharmacol. 2019 Jun 14;10:618. doi: 10.3389/fphar.2019.00618. eCollection 2019.
Bone mesenchymal stem cells (BMSCs) are important candidates for bone regeneration. The role of Bergenin, a C-glucoside of 4-O-methyl gallic acid obtained from the species, Bergenia, in BMSC osteogenesis has not yet been elucidated. We therefore investigated the effects of Bergenin on the osteogenesis of BMSCs and found that Bergenin enhanced osteoblast-specific markers and downregulated the adipocyte-specific markers . Furthermore, using a rat calvarial defect model, we found that Bergenin significantly improved bone healing, as determined by imaging and histological analyses. Moreover, it also upregulated SIRT1 expression. A SIRT1 inhibitor (EX 527) decreased the enhanced bone mineral formation caused by Bergenin. Taken together, these findings show that Bergenin accelerated the osteogenic differentiation of BMSCs, at least partly through the activation of SIRT1.
骨髓间充质干细胞（BMSCs）是骨再生的重要候选细胞。岩白菜素是从岩白菜属植物中提取的4-O-甲基没食子酸的C-葡萄糖苷，其在BMSCs成骨过程中的作用尚未阐明。因此，我们研究了岩白菜素对BMSCs成骨的影响，发现岩白菜素增强了成骨细胞特异性标志物的表达，并下调了脂肪细胞特异性标志物的表达。此外，使用大鼠颅骨缺损模型，我们发现通过影像学和组织学分析确定，岩白菜素显著促进了骨愈合。此外，它还上调了SIRT1的表达。SIRT1抑制剂（EX 527）降低了岩白菜素引起的骨矿物质形成增强。综上所述，这些发现表明岩白菜素至少部分通过激活SIRT1加速了BMSCs的成骨分化。
