Reichl Franz-Xaver, Holdt Lesca Miriam, Teupser Daniel, Schütze Gregor, Metcalfe Alan J, Hickel Reinhard, Högg Christof, Bloch Wilhelm
Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany.
Institute for Laboratory Medicine, Ludwig-Maximilians-Universitat Munchen, Munchen, Germany.
Int J Sports Med. 2017 Oct;38(11):809-818. doi: 10.1055/s-0043-115738. Epub 2017 Sep 11.
The ingredients of Actovegin were analyzed and its effects on the muscle cell proliferation were investigated. C2C12 myoblasts were cultured in medium. Actovegin was added in five different concentrations (1, 5, 25, 125, and 250 µg) to the differentiation medium. The formations of proliferation factor Ki67 and myosin heavy chains were measured by immunofluorescence. The first primary antibody was anti-Ki67 and anti-Mf20. Cells were washed and treated with the second fluorochrome. Thirty-one Actovegin ingredients were found to contain significantly higher concentrations and twenty-nine ingredients were found to contain significantly lower concentrations, compared to the mean ranges as described in the literature for the normal physiological concentrations in human adult serum/plasma. A significant increase in the formation of Ki67 was observed in Actovegin groups, compared to controls. The mean area of myotubes was significantly increased in Actovegin groups. A significant decrease in the number of myotubes was observed. An increased myotube size (fusion) was observed. The intensity of Mf20 was significantly increased in Actovegin groups. It could be demonstrated that Actovegin contains many physiological substances in significantly higher and some in lower concentrations compared to human adult serum. Furthermore, it could be shown that Actovegin improves muscle cell proliferation.
对爱维治的成分进行了分析，并研究了其对肌肉细胞增殖的影响。将C2C12成肌细胞在培养基中培养。在分化培养基中添加五种不同浓度（1、5、25、125和250µg）的爱维治。通过免疫荧光法测定增殖因子Ki67和肌球蛋白重链的形成。第一种一抗是抗Ki67和抗Mf20。细胞洗涤后用第二种荧光染料处理。与文献中描述的成人血清/血浆正常生理浓度的平均范围相比，发现31种爱维治成分的浓度显著更高，29种成分的浓度显著更低。与对照组相比，爱维治组中Ki67的形成显著增加。爱维治组中肌管的平均面积显著增加。观察到肌管数量显著减少。观察到肌管大小（融合）增加。爱维治组中Mf20的强度显著增加。可以证明，与成人血清相比，爱维治含有许多浓度显著更高的生理物质，也有一些浓度更低的。此外，可以证明爱维治能改善肌肉细胞增殖。
