Miyamoto K, Tomita N, Ishii A, Miyamoto N, Nonaka H, Kondo T, Sugihara T, Yawata Y, Tada S, Tsubota T
School of Health Sciences, Okayama University, Japan.
Int J Cancer. 1987 Oct 15;40(4):461-8. doi: 10.1002/ijc.2910400405.
Chromosome analysis was performed on 1 patient with diffuse lymphoma of mixed type by histologic diagnosis and on 7 patients with the acute type of adult T-cell leukemia (ATL). Specific abnormalities in chromosome 14 at break band q11 with the assigned locus of the alpha-chain gene of the T-cell antigen receptor were identified in 6 of 8 patients. Inv(14) (q11q32) was found in 2 patients and translocation of chromosome 14 at break band q11 was observed in 4. Donor chromosomes involved in translocation of the 14q11 varied, i.e., chromosomes 3, 7 or X, with the exception of one patient whose donor chromosome origin could not be determined. The breakpoint in chromosome 3 was in band p25, a region reported to include the locus of the c-raf-I oncogene. In chromosome 7, it was in band p11, a region reported to include the locus of the c-erb-B oncogene, and in the sex chromosome X, it was in band q11. One patient also had a chromosome 14 aberration at break band q32. Of the 2 remaining patients, one had lost chromosome 14 and the other had an isochromosome 14q. Our observation and other reported findings suggest that the rearrangement of chromosome 14 at break band q11 is specific for lymphoma-type or acute-type ATL patients, and aberrations of proto-oncogene expression or the coding sequence by recombination involving a T-cell antigen receptor gene due to chromosome inversion or chromosome translocation may play an important role in T-cell neoplasia including ATL.
对1例经组织学诊断为混合型弥漫性淋巴瘤的患者以及7例急性型成人T细胞白血病（ATL）患者进行了染色体分析。在8例患者中的6例中，发现14号染色体在断裂带q11处存在特异性异常，该断裂带与T细胞抗原受体α链基因的指定位点相关。在2例患者中发现了inv(14)(q11q32)，在4例患者中观察到14号染色体在断裂带q11处发生易位。参与14q11易位的供体染色体各不相同，即3号、7号或X染色体，但有1例患者的供体染色体来源无法确定。3号染色体的断点位于p25带，据报道该区域包含c-raf-I癌基因的位点。在7号染色体中，断点位于p11带，据报道该区域包含c-erb-B癌基因的位点，在性染色体X中，断点位于q11带。1例患者在断裂带q32处也存在14号染色体畸变。在其余2例患者中，1例丢失了14号染色体，另1例有一条等臂染色体14q。我们的观察结果和其他报道的发现表明，14号染色体在断裂带q11处的重排对于淋巴瘤型或急性型ATL患者具有特异性，并且由于染色体倒位或染色体易位导致的涉及T细胞抗原受体基因的重组引起的原癌基因表达或编码序列的畸变可能在包括ATL在内的T细胞肿瘤形成中起重要作用。
