在溶杆菌中激活一个隐匿基因簇揭示了非核糖体肽合成酶在吡咯并吡嗪生物合成中模块/结构域可移动机制。
Activation of a Cryptic Gene Cluster in Lysobacter enzymogenes Reveals a Module/Domain Portable Mechanism of Nonribosomal Peptide Synthetases in the Biosynthesis of Pyrrolopyrazines.
机构信息
State Key Laboratory of Microbial Technology, College of Life Sciences, Shandong University , Jinan 250100, China.
College of Pharmacy, Ningxia Medical University , Yinchuan 750004, China.
出版信息
Org Lett. 2017 Oct 6;19(19):5010-5013. doi: 10.1021/acs.orglett.7b01611. Epub 2017 Sep 12.
Abstract
Lysobacter are considered "peptide specialists". However, many of the nonribosomal peptide synthetase genes are silent. Three new compounds were identified from L. enzymogenes upon activating the six-module-containing led cluster by the strong promoter P. Although ledD was the first gene under P control, the second gene ledE was expressed the highest. Targeted gene inactivation showed that the two-module LedE and the one-module LedF were selectively used in pyrrolopyrazine biosynthesis, revealing a module/domain portable mechanism.
摘要
Lysobacter 被认为是“肽类专家”。然而，许多非核糖体肽合成酶基因是沉默的。通过强启动子 P 激活含有六个模块的 led 簇，从 L. enzymogenes 中鉴定出三种新化合物。尽管 ledD 是 P 控制下的第一个基因，但第二个基因 ledE 的表达量最高。靶向基因失活表明，两个模块的 LedE 和一个模块的 LedF 被选择性地用于吡咯并吡嗪的生物合成，揭示了一种模块/结构域可移动的机制。
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