Barreto-Vieira Debora Ferreira, Jácome Fernanda Cunha, da Silva Marcos Alexandre Nunes, Caldas Gabriela Cardoso, de Filippis Ana Maria Bispo, de Sequeira Patrícia Carvalho, de Souza Elen Mello, Andrade Audrien Alves, Manso Pedro Paulo de Abreu, Trindade Gisela Freitas, Lima Sheila Maria Barbosa, Barth Ortrud Monika
Laboratory of Morphology and Viral Morphogenesis, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil, Rio de Janeiro, RJ, Brazil.
Laboratory of Flavivirus, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil, Rio de Janeiro, RJ, Brazil.
PLoS One. 2017 Sep 12;12(9):e0184397. doi: 10.1371/journal.pone.0184397. eCollection 2017.
Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.
寨卡病毒（ZIKV）是黄病毒属的一员，其基因组是约10.8千碱基的正链RNA，包裹在衣壳中并被膜包围。关于寨卡病毒复制动力学的研究很少，这限制了针对寨卡病毒的抗病毒药物和疫苗的开发。在本研究中，用从一名巴西患者分离出的寨卡病毒样本接种白纹伊蚊细胞系（C6/36细胞）和非洲绿猴肾上皮细胞（Vero细胞），并通过免疫荧光染色、相差光学显微镜、透射电子显微镜和实时逆转录聚合酶链反应对感染进行表征。在这两种细胞系中均观察到感染，并且在溶酶体、粗面内质网和病毒质样结构内观察到寨卡病毒颗粒。证实了C6/36和Vero细胞对寨卡病毒感染的易感性。此外，本研究表明部分复制周期可能发生在病毒质样结构内，这在其他黄病毒中尚未得到证实。
