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巴西的登革热、黄热病、寨卡病毒病和基孔肯雅热流行虫媒病毒：超微结构方面

Dengue, Yellow Fever, Zika and Chikungunya epidemic arboviruses in Brazil: ultrastructural aspects.

作者信息

Barreto-Vieira Debora Ferreira, Couto-Lima Dinair, Jácome Fernanda Cunha, Caldas Gabriela Cardoso, Barth Ortrud Monika

机构信息

Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil.

Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Mosquitos Transmissores de Hematozoários, Rio de Janeiro, RJ, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2021 Feb 3;115:e200278. doi: 10.1590/0074-02760200278. eCollection 2021.

DOI:10.1590/0074-02760200278
PMID:33566939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7860610/
Abstract

BACKGROUND

The impact of arbovirus cocirculation in Brazil is unknown. Dengue virus (DENV) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. The Zika virus (ZIKV) epidemic in the Americas provided pathogenicity evidence that had not been previously observed in flavivirus infections. In contrast to other flaviviruses, electron microscopy studies have shown that ZIKV may replicate in viroplasm-like structures. Flaviviruses produce an ensemble of structurally different virions, collectively contributing to tissue tropism and virus dissemination.

OBJECTIVES AND METHODS

In this work, the Aedes albopictus mosquito cell lineage (C6/36 cells) and kidney epithelial cells from African green monkeys (Vero cells) were infected with samples of the main circulating arboviruses in Brazil [DENV-1, DENV-2, DENV-3, DENV-4, ZIKV, Yellow Fever virus (YFV) and Chikungunya virus (CHIKV)], and ultrastructural studies by transmission electron microscopy were performed.

FINDINGS

We observed that ZIKV, the DENV serotypes, YFV and CHIKV particles are spherical. ZIKV, DENV-1, -2, -3 and -4 presented diameters of 40-50 nm, and CHIKV presented approximate diameters of 50-60 nm. Viroplasm-like structures was observed in ZIKV replication cycle.

MAIN CONCLUSIONS

The morphogenesis of these arboviruses is similar to what has been presented in previous studies. However, we understand that further studies are needed to investigate the relationship between viroplasm-like structures and ZIKV replication dynamics.

摘要

背景

虫媒病毒在巴西共同传播的影响尚不清楚。登革病毒（DENV）再次感染可能导致更强的病毒血症或免疫病理反应，从而引发更严重的疾病。美洲的寨卡病毒（ZIKV）疫情提供了黄病毒感染中以前未观察到的致病性证据。与其他黄病毒不同，电子显微镜研究表明ZIKV可能在病毒质样结构中复制。黄病毒产生一组结构不同的病毒粒子，共同影响组织嗜性和病毒传播。

目的和方法

在这项研究中，用巴西主要流行的虫媒病毒样本[DENV-1、DENV-2、DENV-3、DENV-4、ZIKV、黄热病毒（YFV）和基孔肯雅病毒（CHIKV）]感染白纹伊蚊细胞系（C6/36细胞）和非洲绿猴肾上皮细胞（Vero细胞），并通过透射电子显微镜进行超微结构研究。

研究结果

我们观察到ZIKV、DENV各血清型、YFV和CHIKV粒子均为球形。ZIKV、DENV-1、-2、-3和-4的直径为40-50nm，CHIKV的直径约为50-60nm。在ZIKV复制周期中观察到病毒质样结构。

主要结论

这些虫媒病毒的形态发生与先前研究的结果相似。然而，我们认为需要进一步研究来探讨病毒质样结构与ZIKV复制动力学之间的关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/2395c6b5af71/1678-8060-mioc-115-e200278-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/7c9ddbb89295/1678-8060-mioc-115-e200278-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/c15173e62479/1678-8060-mioc-115-e200278-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/e85c0db0e9b8/1678-8060-mioc-115-e200278-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/3482086149e7/1678-8060-mioc-115-e200278-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/5efb3d7abda7/1678-8060-mioc-115-e200278-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/2395c6b5af71/1678-8060-mioc-115-e200278-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/7c9ddbb89295/1678-8060-mioc-115-e200278-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/c15173e62479/1678-8060-mioc-115-e200278-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/e85c0db0e9b8/1678-8060-mioc-115-e200278-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/3482086149e7/1678-8060-mioc-115-e200278-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/5efb3d7abda7/1678-8060-mioc-115-e200278-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5e0/7860610/2395c6b5af71/1678-8060-mioc-115-e200278-gf6.jpg

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