Zhao Huanyu, Zhang Di, Yang Lianhe, Wang Enhua
Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China.
Mol Carcinog. 2018 Jan;57(1):89-96. doi: 10.1002/mc.22734. Epub 2017 Sep 25.
As a member of the p120-catenin (p120ctn) subfamily, the p0071 study in tumor is very limited. We demonstrated the clinicopathological significance of p0071 in non-small cell lung cancer (NSCLC), as well as E-cadherin. Co-immunoprecipitation was used to detect the interaction of p0071 with E-cadherin in A549 and SPC cells (E-cadherin is mainly expressed in the cytoplasm of these cells). p0071 cytoplasmic expression was knocked down by siRNA in these cells and this effect on the RhoA activity and cell invasion and migration ability were measured. p0071 overexpression in the cytoplasm of tumor cell was correlated with lymphatic metastase and poor prognosis of NSCLC. The patients with both abnormal expression of p0071 and E-cadherin (cytoplasmic expression) had a statistically significant shorter survival than the patients without both abnormal expression (P < 0.05). There is a significant correlation between cytoplasmic overexpression of p0071 and E-cadherin in NSCLC tissues. p0071 interacted with E-cadherin in the cytoplasm of A549 and SPC cell lines. Treatment with siRNA-p0071 inhibited the invasion and migration ability of NSCLC cells. Above results confirmed that p0071 interacted with E-cadherin in the cytoplasm so as to promote the invasion and metastasis of NSCLC.
作为p120连环蛋白（p120ctn）亚家族的一员，p0071在肿瘤方面的研究非常有限。我们阐述了p0071以及E-钙黏蛋白在非小细胞肺癌（NSCLC）中的临床病理意义。采用免疫共沉淀法检测p0071与A549和SPC细胞中E-钙黏蛋白的相互作用（E-钙黏蛋白主要在这些细胞的细胞质中表达）。在这些细胞中用小干扰RNA（siRNA）敲低p0071的细胞质表达，并检测其对RhoA活性以及细胞侵袭和迁移能力的影响。肿瘤细胞细胞质中p0071的过表达与NSCLC的淋巴转移和不良预后相关。p0071和E-钙黏蛋白（细胞质表达）均异常的患者，其生存期在统计学上显著短于两者均无异常表达的患者（P<0.05）。NSCLC组织中p0071和E-钙黏蛋白的细胞质过表达之间存在显著相关性。在A549和SPC细胞系的细胞质中，p0071与E-钙黏蛋白相互作用。用siRNA-p0071处理可抑制NSCLC细胞的侵袭和迁移能力。上述结果证实，p0071在细胞质中与E-钙黏蛋白相互作用，从而促进NSCLC的侵袭和转移。
