School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, PR China.
School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, PR China.
Bioresour Technol. 2017 Dec;245(Pt A):518-529. doi: 10.1016/j.biortech.2017.08.102. Epub 2017 Aug 19.
The green microalga Chromochloris zofingiensis is a promising producer of natural astaxanthin. In the present study, C. zofingiensis was first cultivated in shake flasks under low light irradiation and then subjected to continuous high light irradiation, which effectively promoted astaxanthin production. In addition, a microplate-based culture system in concert with high light irradiation from blue light and white light above 150μmolms was constructed and applied to improve astaxanthin production. Blue light exerted more positive influences on astaxanthin accumulation, but when the light intensity was increased to 300μmolms, astaxanthin biosynthesis was substantially inhibited. Conversely, in a nitrogen-deprived culture under white light, the highest astaxanthin content for C. zofingiensis, 7.1mg/g, was obtained. The highest astaxanthin yield achieved was 38.9mg/L in a culture with 0.1g/L nitrate under the same culture conditions. This study demonstrates that C. zofingiensis has great potential for natural astaxanthin production.
产天然虾青素的绿藻雨生红球藻的培养条件优化。本研究首先在摇瓶中低光下培养雨生红球藻，然后进行连续高光照射，有效促进了虾青素的生产。此外，构建了一种基于微孔板的培养系统，并结合蓝光和白光（强度大于 150μmolms）进行连续光照，以提高虾青素的产量。蓝光对虾青素的积累有更积极的影响，但当光强度增加到 300μmolms 时，虾青素的生物合成受到了很大抑制。相反，在缺氮的白光培养条件下，雨生红球藻获得了最高的虾青素含量，为 7.1mg/g。在相同的培养条件下，在含 0.1g/L 硝酸盐的培养物中，虾青素的最高产量达到 38.9mg/L。本研究表明，雨生红球藻具有生产天然虾青素的巨大潜力。
